Publication: Buffer-optimized high gradient magnetic separation: Target cell capture efficiency is predicted by linear bead-capture theory
Issued Date
2016-01-01
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ISSN
12261750
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2-s2.0-84962120864
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Magnetics. Vol.21, No.1 (2016), 125-132
Suggested Citation
Shahid Waseem, Rachanee Udomsangpetch, Sebastian C. Bhakdi Buffer-optimized high gradient magnetic separation: Target cell capture efficiency is predicted by linear bead-capture theory. Journal of Magnetics. Vol.21, No.1 (2016), 125-132. doi:10.4283/JMAG.2016.21.1.125 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/40609
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Title
Buffer-optimized high gradient magnetic separation: Target cell capture efficiency is predicted by linear bead-capture theory
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Abstract
© The Korean Magnetics Society. All rights reserved. High gradient magnetic separation (HGMS) is the most commonly used magnetic cell separation technique in biomedical science. However, parameters determining target cell capture efficiencies in HGMS are still not well understood. This limitation leads to loss of information and resources. The present study develops a beadcapture theory to predict capture efficiencies in HGMS. The theory is tested with CD3- and CD14-positive cells in combination with paramagnetic beads of different sizes and a generic immunomagnetic separation system. Data depict a linear relationship between normalized capture efficiency and the bead concentration. In addition, it is shown that key biological functions of target cells are not affected for all bead sizes and concentrations used. In summary, linear bead-capture theory predicts capture efficiency (Et) in a highly significant manner.