Identification of Cryptosporidium parvum genotype from HIV and non-HIV fecal samples by PCR

Abstract

In this study, specific primers of genotypes 1 and 2 were used to identify Cryptosporidium parvum genotypes in fecal samples. A total of 30 fecal HIV and non-HIV samples of C. parvum were examined by microscopic method, comprised of 7 samples from non-HIV children aged 8-12 years, 11 fecal samples from HIV-positive adults and 12 purified oocysts of C. parvum from HIV patients. Within this group of infected children, 5 were infected with genotype 1, while 2 samples were unclassified. In the HIV-positive adult patients, 7 samples were genotype 1, while 4 samples were unclassified. Of the 12 purified oocyst samples, 11 samples were positive for genotype 1, while only 1 purified oocyst sample was unclassified. The unclassified samples observed in our study may belong to other genotypes and no C. parvum genotype 2 was detected in our study population.