Publication: Detection and inhibition of lipid-derived radicals in low-density lipoprotein
Issued Date
2017-12-01
Resource Type
ISSN
18734596
08915849
08915849
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2-s2.0-85032684862
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Mahidol University
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SCOPUS
Bibliographic Citation
Free Radical Biology and Medicine. Vol.113, (2017), 487-493
Suggested Citation
Yuma Ishida, Yuka Okamoto, Yuta Matsuoka, Arisa Tada, Jindaporn Janprasit, Mayumi Yamato, Noppawan Phumala Morales, Ken Ichi Yamada Detection and inhibition of lipid-derived radicals in low-density lipoprotein. Free Radical Biology and Medicine. Vol.113, (2017), 487-493. doi:10.1016/j.freeradbiomed.2017.10.388 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/41689
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Title
Detection and inhibition of lipid-derived radicals in low-density lipoprotein
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Abstract
© 2017 Elsevier Inc. Oxidized low density lipoprotein (Ox-LDL) is implicated in a variety of oxidative diseases. To clarify the mechanisms involved and facilitate the investigation of therapeutics, we previously developed a detection method for lipid-derived radicals using the fluorescent probe 2,2,6-trimethyl-6-pentyl-4-(4-nitrobenzo[1,2,5]oxadiazol-7-ylamino)piperidine-1-oxyl (NBD-Pen). In this study, NBD-Pen was used to detect lipid-derived radicals in Ox-LDL from in vitro and in vivo samples using an iron overloaded mouse model. By following the timeline of lipid radical generation using this method, the iron overloaded mice could be successfully treated with the antioxidant Trolox, resulting in successful lowering of the plasma lipid peroxidation, aspartate transaminase and alanine transaminase levels. Furthermore, using a combination therapy of the chelating agent deferoxamine (DFX) and Trolox, liver injury and oxidative stress markers were also reduced in iron overloaded mice. The NBD-Pen method is highly sensitive as well as selective and is suitable for targeting minimally modified LDL compared with other existing methods.