Publication: Inhibition of HMGB1 translocation by green tea extract in rats exposed to environmental tobacco smoke
Issued Date
2012-01-01
Resource Type
ISSN
19061714
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2-s2.0-82755176341
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Mahidol University
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SCOPUS
Bibliographic Citation
EnvironmentAsia. Vol.5, No.1 (2012), 70-76
Suggested Citation
Sirintip Chaichalotornkul, Wisuda Suvitayavat, Vanida Sangalangkarn, Yuko Nawa, Kiyoshi Kikuchi, Koichi Kawahara, Tawee Saiwichai, Somphong Narkpinit, Pratap Singhasivanon, Ikuro Maruyama, Salunya Tancharoen Inhibition of HMGB1 translocation by green tea extract in rats exposed to environmental tobacco smoke. EnvironmentAsia. Vol.5, No.1 (2012), 70-76. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/14208
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Title
Inhibition of HMGB1 translocation by green tea extract in rats exposed to environmental tobacco smoke
Abstract
Environmental tobacco smoke (ETS) exposure is linked to carcinogenic, oxidative and inflammatory cellular reactions. Green tea polyphenol reportedly plays a role in the prevention of inflammation-related diseases. To evaluate the effects of green tea extract (GTE) on cellular location of High Mobility Group Box-1 (HMGB1) protein, we studied the lung tissue in rats exposed to cigarette smoke (CS). Rats were divided into three groups; CS, CSG, and C, which were groups of CS-treated only, CS-treated with GTE dietary supplement, and the control, respectively. Our findings by immunocytochemistry showed that abundant HMGB1 translocated from the nucleus to the cytoplasm in the lung tissues of rats that were exposed to CS, whereas HMGB1 was localized to the nuclei of CSG and C group. For in vitro studies, cotinine stimulated the secretion of HMGB1 in a dose and time dependent manner and the HMGB1 level was suppressed by GTE in murine macrophage cell lines. Our results could suggest that GTE supplementation which could suppress HMGB1 may offer a beneficial effect against diseases.