Publication: Immunohistochemical expression of type II collagen in the lingual mucosa of rats during organogenesis of the tongue
Issued Date
2008-07-01
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ISSN
00039969
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2-s2.0-43049183581
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Mahidol University
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SCOPUS
Bibliographic Citation
Archives of Oral Biology. Vol.53, No.7 (2008), 622-628
Suggested Citation
Tomoichiro Asami, Hidekazu Aoyagi, Hideki Yoshizawa, Chaitip Wanichanon, Shin ichi Iwasaki Immunohistochemical expression of type II collagen in the lingual mucosa of rats during organogenesis of the tongue. Archives of Oral Biology. Vol.53, No.7 (2008), 622-628. doi:10.1016/j.archoralbio.2008.01.010 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/19161
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Title
Immunohistochemical expression of type II collagen in the lingual mucosa of rats during organogenesis of the tongue
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Abstract
Objectives: We examined the timing of the appearance and distribution of type II collagen as a possible component of the extracellular matrix that is involved in the morphogenesis of the rat tongue. Methods: We examined the immunofluorescence of type II collagen, differential interference contrast (DIC) images, and images recorded in transmission mode after toluidine blue staining by laser-scanning microscopy (LSM) during the morphogenesis of filiform papillae and the keratinization of the lingual epithelium of rats on semi-ultrathin sections of epoxy resin-embedded samples. Results: Immunoreactivity specific for type II collagen was scattered on cells over a wide area of the mesenchymal connective tissue of the fetal tongue on day 15 after conception (E15), when the lingual epithelium was composed of one or two layers of cuboidal cells. Immunoreactivity specific for type II collagen was recognisable on cells of the lamina propria of the lingual mucosa and around the developing lingual muscle of fetuses at E17 and E19. On E19, the epithelium was clearly of the stratified squamous type. At postnatal stages after birth (P0), immunoreactivity became more and more significant in the connective tissue of the lamina propria with the advancing of morphogenesis of the filiform papillae. In addition, immunoreactivity was widely distributed in the connective tissue around the lingual muscle, as myogenesis in the tongue advanced. The lingual epithelium was composed of stratified squamous cells, and keratinization of the lingual epithelium proceeded gradually as morphogenesis of filiform papillae continued during postnatal development. Conclusion: Type II collagen appeared not only in the connective tissue of the lamina propria as the morphogenesis of filiform papillae occurred and the lingual epithelium became keratinized but also in the endomysium and perimysium around the lingual muscle after myogenesis of the tongue is complete at P0. © 2008 Elsevier Ltd. All rights reserved.