Publication:
Transcriptome and histone epigenome of Plasmodium vivax salivary-gland sporozoites point to tight regulatory control and mechanisms for liver-stage differentiation in relapsing malaria

Issued Date

2019-06-01

Resource Type

Article

ISSN

18790135
00207519

DOI

10.1016/j.ijpara.2019.02.007

Other identifier(s)

2-s2.0-85065546135

Rights

Mahidol University

Rights Holder(s)

SCOPUS

Bibliographic Citation

International Journal for Parasitology. Vol.49, No.7 (2019), 501-513

Suggested Citation

Ivo Muller, Aaron R. Jex, Stefan H.I. Kappe, Sebastian A. Mikolajczak, Jetsumon Sattabongkot, Rapatbhorn Patrapuvich, Scott Lindner, Erika L. Flannery, Cristian Koepfli, Brendan Ansell, Anita Lerch, Samantha J. Emery-Corbin, Sarah Charnaud, Jeffrey Smith, Nicolas Merrienne, Kristian E. Swearingen, Robert L. Moritz, Michaela Petter, Michael F. Duffy, Vorada Chuenchob Transcriptome and histone epigenome of Plasmodium vivax salivary-gland sporozoites point to tight regulatory control and mechanisms for liver-stage differentiation in relapsing malaria. International Journal for Parasitology. Vol.49, No.7 (2019), 501-513. doi:10.1016/j.ijpara.2019.02.007 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/51063

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Title

Transcriptome and histone epigenome of Plasmodium vivax salivary-gland sporozoites point to tight regulatory control and mechanisms for liver-stage differentiation in relapsing malaria

Author(s)

Ivo Muller
 Aaron R. Jex
 Stefan H.I. Kappe
 Sebastian A. Mikolajczak
 Jetsumon Sattabongkot
 Rapatbhorn Patrapuvich
 Scott Lindner
 Erika L. Flannery
 Cristian Koepfli
 Brendan Ansell
 Anita Lerch
 Samantha J. Emery-Corbin
 Sarah Charnaud
 Jeffrey Smith
 Nicolas Merrienne
 Kristian E. Swearingen
 Robert L. Moritz
 Michaela Petter
 Michael F. Duffy
 Vorada Chuenchob

Other Contributor(s)

Faculty of Veterinary and Agricultural Sciences
 Universitätsklinik Erlangen und Medizinische Fakultät
 Walter and Eliza Hall Institute of Medical Research
 University of Melbourne
 Children's Hospital and Regional Medical Center
 University of Washington, Seattle
 Mahidol University
 Institute for Systems Biology
 Institut Pasteur, Paris
 Pennsylvania State University

Abstract

© 2019 Plasmodium vivax is the key obstacle to malaria elimination in Asia and Latin America, largely attributed to its ability to form resilient hypnozoites (sleeper cells) in the host liver that escape treatment and cause relapsing infections. The decision to form hypnozoites is made early in the liver infection and may already be set in sporozoites prior to invasion. To better understand these early stages of infection, we undertook a comprehensive transcriptomic and histone epigenetic characterization of P. vivax sporozoites. Through comparisons with recently published proteomic data for the P. vivax sporozoite, our study found that although highly transcribed, transcripts associated with functions needed for early infection of the vertebrate host are not detectable as proteins and may be regulated through translational repression. We identified differential transcription between the sporozoite and published transcriptomes of asexual blood stages and mixed versus hypnozoite-enriched liver stages. These comparisons point to multiple layers of transcriptional, post-transcriptional and post-translational control that appear active in sporozoites and to a lesser extent hypnozoites, but are largely absent in replicating liver schizonts or mixed blood stages. We also characterised histone epigenetic modifications in the P. vivax sporozoite and explored their role in regulating transcription. Collectively, these data support the hypothesis that the sporozoite is a tightly programmed stage to infect the human host and identify mechanisms for hypnozoite formation that may be further explored in liver stage models.

Keyword(s)

Immunology and Microbiology
 Medicine

Availability

URI

https://repository.li.mahidol.ac.th/handle/20.500.14594/51063

Collections

Scopus 2019