Publication: Direct assay of bound sialic acids on rat spermatozoa from the caput and cauda epididymidis
Issued Date
1983-01-01
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ISSN
00224251
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2-s2.0-0020530318
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Reproduction and Fertility. Vol.67, No.2 (1983), 275-280
Suggested Citation
P. Toowicharanont, M. Chulavatnatol Direct assay of bound sialic acids on rat spermatozoa from the caput and cauda epididymidis. Journal of Reproduction and Fertility. Vol.67, No.2 (1983), 275-280. doi:10.1530/jrf.0.0670275 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/30456
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Title
Direct assay of bound sialic acids on rat spermatozoa from the caput and cauda epididymidis
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Abstract
Bound sialic acids on rat spermatozoa were assayed by oxidation with 1 mM-NaIO4at 0°C, liberating C-9 as formaldehyde which was further quantitated using 3-methyl-2-benzothiazolinone. The mean ± s.d. (n=20) content of bound sialic acids of spermatozoa from the caput and cauda epididymidis was 50.9 ± 8.0 and 25.2 ± 3.8 nmol/108spermatozoa respectively. About 85% of the former and 75% of the latter could be extracted by 1% Triton X-100 and 2 mM-dithiothreitol. About 70% of the former and 20% of the latter were released by neuraminidase from Vibrio cholerae. About 40% of the former and 30% of the latter were sensitive to trypsin. During sperm maturation, the decrease in the total bound sialic acids was due to the decrease in the neuraminidase-sensitive but not the neuraminidase-resistant sialic acids.