Publication: Subcellular localizations and time-course expression of dengue envelope and non-structural 1 proteins in human endothelial cells
| dc.contributor.author | Jesdaporn Poungsawai | en_US |
| dc.contributor.author | Rattiyaporn Kanlaya | en_US |
| dc.contributor.author | Sa nga Pattanakitsakul | en_US |
| dc.contributor.author | Visith Thongboonkerd | en_US |
| dc.contributor.other | Mahidol University | en_US |
| dc.date.accessioned | 2018-05-03T08:15:12Z | |
| dc.date.available | 2018-05-03T08:15:12Z | |
| dc.date.issued | 2011-09-01 | en_US |
| dc.description.abstract | We performed subcellular fractionation of human endothelial cells (EA.hy926) after a challenge with dengue virus serotype-2 (DEN-2) at multiplicity of infection (MOI) of 10 for 2 h. The cells were fractionated into four fractions including cytosol, membrane/organelle, nucleus and cytoskeleton, and their purity was nicely confirmed by immunofluorescence staining of markers for individual subcellular compartments. Western blot analysis of dengue envelope (E) and non-structural 1 (NS1) proteins showed that E was present only in membrane/organelle fraction, whereas NS1 was present in membrane/organelle, nucleus, and cytoskeleton fractions. Time-course study showed that E was present immediately after the challenge, whereas NS1 was present after 12 h of the challenge. Our data may provide a new roadmap for studying mechanisms of replication of dengue virus and its interactions with host cells. © 2011 Elsevier Ltd. | en_US |
| dc.identifier.citation | Microbial Pathogenesis. Vol.51, No.3 (2011), 225-229 | en_US |
| dc.identifier.doi | 10.1016/j.micpath.2011.04.011 | en_US |
| dc.identifier.issn | 10961208 | en_US |
| dc.identifier.issn | 08824010 | en_US |
| dc.identifier.other | 2-s2.0-79959335103 | en_US |
| dc.identifier.uri | https://repository.li.mahidol.ac.th/handle/123456789/11999 | |
| dc.rights | Mahidol University | en_US |
| dc.rights.holder | SCOPUS | en_US |
| dc.source.uri | https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=79959335103&origin=inward | en_US |
| dc.subject | Immunology and Microbiology | en_US |
| dc.subject | Medicine | en_US |
| dc.title | Subcellular localizations and time-course expression of dengue envelope and non-structural 1 proteins in human endothelial cells | en_US |
| dc.type | Article | en_US |
| dspace.entity.type | Publication | |
| mu.datasource.scopus | https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=79959335103&origin=inward | en_US |