Publication: Molecular and serological survey on taeniasis and cysticercosis in Kanchanaburi Province, Thailand
Issued Date
2010-09-01
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ISSN
13835769
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2-s2.0-77955326461
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Mahidol University
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SCOPUS
Bibliographic Citation
Parasitology International. Vol.59, No.3 (2010), 326-330
Suggested Citation
Malinee T. Anantaphruti, Munehiro Okamoto, Tippayarat Yoonuan, Surapol Saguankiat, Teera Kusolsuk, Megumi Sato, Marcello O. Sato, Yasuhito Sako, Jitra Waikagul, Akira Ito Molecular and serological survey on taeniasis and cysticercosis in Kanchanaburi Province, Thailand. Parasitology International. Vol.59, No.3 (2010), 326-330. doi:10.1016/j.parint.2010.03.007 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/29197
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Title
Molecular and serological survey on taeniasis and cysticercosis in Kanchanaburi Province, Thailand
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Abstract
A community-based field survey on taeniasis and cysticercosis was performed in two villages in Thong Pha Phum District, Kanchanaburi Province, central Thailand, where 3 Taenia species, T. solium, T. saginata and T. asiatica, are sympatrically occurring. Four (0.6%) out of 667 stool samples were egg-positive for Taenia sp. by Kato-Katz technique. Three out of those four persons and other three persons who were Taenia egg-negative but having a recent (<1 year) history of discharging worms in stool were treated with niclosamide. One Taenia egg-positive woman was not treated because of severe ascites. After treatment, three persons expelled long strobilae with scolices and two persons expelled strobilae without scolex. One Taenia egg-positive person did not expel any worms post-treatment. Among 5 persons, four expelled a single worm, whereas one expelled multiple worms, may be 6 worms but not confirmed by detection of scolices. One scolex was armed with hooklets, whereas 2 others did not. Multiplex PCR of 10 expelled proglottids (including 6 estimated worms from one patient) revealed that one sample was T. solium, one T. saginata, and 8 T. asiatica. A total of 159 residents agreed to receive a serological test for cysticercosis. By ELISA using partially purified glycoprotein antigen, 9 cases, 5 and 4 from villages A and B respectively, were found to be sero-positive. The five and an additional sample on the border line from village A were evaluated using confirmative immunoblot using recombinant chimeric antigen. Among the six samples, four including the border line sample were confirmed to be cysticercosis by immunoblotting. One of the 4 persons had neurological symptoms with nodular lesions in the brain by computed tomography. These 4 confirmed or suspected cysticercosis cases were free of T. solium worms, but two of them including confirmed NCC case had a past (>1 year) history of expelling proglottids in the stool. © 2010.