Publication: Molecular cloning and characterization of cathepsin L encoding genes from Fasciola gigantica
Issued Date
2001-07-12
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ISSN
13835769
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2-s2.0-0034950247
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Mahidol University
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SCOPUS
Bibliographic Citation
Parasitology International. Vol.50, No.2 (2001), 105-114
Suggested Citation
Rudi Grams, Suksiri Vichasri-Grams, Prasert Sobhon, E. Suchart Upatham, Vithoon Viyanant Molecular cloning and characterization of cathepsin L encoding genes from Fasciola gigantica. Parasitology International. Vol.50, No.2 (2001), 105-114. doi:10.1016/S1383-5769(01)00068-X Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/26562
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Title
Molecular cloning and characterization of cathepsin L encoding genes from Fasciola gigantica
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Abstract
In this study cDNAs encoding cathepsin L-like proteins of Fasciola gigantica were cloned by the reverse transcription polymerase chain reaction method (RT-PCR) from total RNA of adult specimens. DNA sequence analyses revealed that six different cathepsin L cDNA fragments were isolated, which have DNA sequence identities of 87-99% towards the homologous genes from F. hepatica. Gene expression was studied at the RNA level by Northern and RNA in situ hybridizations. Northern analysis showed the cathepsin L genes to be strongly expressed in adult parasites as a group of 1050 nt sized RNAs. RNA in situ hybridization localized cathepsin L RNA to the cecal epithelial cells. Southern hybridization was used to determine the number of cathepsin L genes and indicated the presence of a family of closely related cathepsin L genes in the genome of F. gigantica. Copyright © 2001 Elsevier Science Ireland Ltd.