Publication: Peganum harmala extract has antiamoebic activity to acanthamoeba triangularis trophozoites and changes expression of autophagy-related genes
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Issued Date
2021-07-01
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ISSN
20760817
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2-s2.0-85110369840
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Mahidol University
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SCOPUS
Bibliographic Citation
Pathogens. Vol.10, No.7 (2021)
Suggested Citation
Rachasak Boonhok, Suthinee Sangkanu, Julalak Chuprom, Mayuna Srisuphanunt, Roghayeh Norouzi, Abolghasem Siyadatpanah, Farzaneh Mirzaei, Watcharapong Mitsuwan, Sueptrakool Wisessombat, Maria de Lourdes Pereira, Mohammed Rahmatullah, Polrat Wilairatana, Christophe Wiart, Lim Chooi Ling, Karma G. Dolma, Veeranoot Nissapatorn Peganum harmala extract has antiamoebic activity to acanthamoeba triangularis trophozoites and changes expression of autophagy-related genes. Pathogens. Vol.10, No.7 (2021). doi:10.3390/pathogens10070842 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/76116
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Title
Peganum harmala extract has antiamoebic activity to acanthamoeba triangularis trophozoites and changes expression of autophagy-related genes
Author(s)
Rachasak Boonhok
Suthinee Sangkanu
Julalak Chuprom
Mayuna Srisuphanunt
Roghayeh Norouzi
Abolghasem Siyadatpanah
Farzaneh Mirzaei
Watcharapong Mitsuwan
Sueptrakool Wisessombat
Maria de Lourdes Pereira
Mohammed Rahmatullah
Polrat Wilairatana
Christophe Wiart
Lim Chooi Ling
Karma G. Dolma
Veeranoot Nissapatorn
Suthinee Sangkanu
Julalak Chuprom
Mayuna Srisuphanunt
Roghayeh Norouzi
Abolghasem Siyadatpanah
Farzaneh Mirzaei
Watcharapong Mitsuwan
Sueptrakool Wisessombat
Maria de Lourdes Pereira
Mohammed Rahmatullah
Polrat Wilairatana
Christophe Wiart
Lim Chooi Ling
Karma G. Dolma
Veeranoot Nissapatorn
Other Contributor(s)
Faculty of Tropical Medicine, Mahidol University
Sikkim Manipal Institute of Medical Sciences
The University of Nottingham Malaysia Campus
Walailak University
Birjand University of Medical Sciences
CICECO – Instituto de Materiais de Aveiro
Shahid Sadoughi University of Medical Sciences
University of Tabriz
International Medical University
University of Development Alternative
Sikkim Manipal Institute of Medical Sciences
The University of Nottingham Malaysia Campus
Walailak University
Birjand University of Medical Sciences
CICECO – Instituto de Materiais de Aveiro
Shahid Sadoughi University of Medical Sciences
University of Tabriz
International Medical University
University of Development Alternative
Abstract
Peganum harmala, a well-known medicinal plant, has been used for several therapeutic purposes as it contains numerous pharmacological active compounds. Our study reported an anti-parasitic activity of P. harmala seed extract against Acanthamoeba triangularis. The stress induced by the extract on the surviving trophozoites for Acanthamoeba encystation and vacuolization was examined by microscopy, and transcriptional expression of Acanthamoeba autophagy-related genes was investigated by quantitative PCR. Our results showed that the surviving trophozoites were not transformed into cysts, and the number of trophozoites with enlarged vacuoles were not significantly different from that of untreated control. Molecular analysis data demonstrated that the mRNA expression of tested AcATG genes, i.e., ATG3, ATG8b, and ATG16, was at a basal level along the treatment. However, upregulation of AcATG16 at 24 h post treatment was observed, which may indicate an autophagic activity of this protein in response to the stress. Altogether, these data revealed the anti-Acanthamoeba activity of P. harmala extract and indicated the association of autophagy mRNA expression and cyst formation under the extract stress, representing a promising plant for future drug development. However, further identification of an active compound and a study of autophagy at the protein level are needed.