Publication: Differentiation of subtypes B and E of human immunodeficiency virus type 1 by polymerase chain reaction using novel env gene primers
Issued Date
2002-02-28
Resource Type
ISSN
01660934
Other identifier(s)
2-s2.0-0036176494
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Virological Methods. Vol.101, No.1-2 (2002), 11-20
Suggested Citation
Fumihiro Yagyu, Yusei Ikeda, Koya Ariyoshi, Wataru Sugiura, Som Arch Wongkhomthong, Michiaki Masuda, Hiroshi Ushijima Differentiation of subtypes B and E of human immunodeficiency virus type 1 by polymerase chain reaction using novel env gene primers. Journal of Virological Methods. Vol.101, No.1-2 (2002), 11-20. doi:10.1016/S0166-0934(01)00415-3 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/20218
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Title
Differentiation of subtypes B and E of human immunodeficiency virus type 1 by polymerase chain reaction using novel env gene primers
Abstract
Novel sets of env gene PCR primers for distinguishing human immunodeficiency virus type 1 (HIV-1) subtypes B and E were designed. These primers anneal to different regions of the env gene and amplify DNA fragments of distinct sizes in a subtype-specific manner. Blood samples from 11 HIV-1 carriers in Thailand and 46 carriers in Japan were examined by PCR. The new env primers detected HIV-1 proviral DNA in 100% (11/11) and 88% (37/42) of the subtype B and E infection cases, respectively. The env primers also detected proviral DNA in saliva and breast milk samples in seven of 11 cases and two of three cases, respectively. The PCR subtyping results matched completely with those obtained by nucleotide sequencing of the env V3 region. The results suggest that the PCR using the env primers designed in this study may be an accurate and cost-effective method for differentiating subtypes B and E of HIV-1 in a large number of clinical samples. However, subtype E specific primer cross-react with subtype A, C, G, the new primer in this study is useful for regions in South East Asia where subtype E is predominant. © 2002 Elsevier Science B.V. All rights reserved.