Publication: Vasorelaxant and Antioxidant Activities of Spilanthes acmella Murr
Submitted Date
2008-12-18
Accepted Date
2008-06-12
Issued Date
2008
Resource Type
Language
eng
Rights
Mahidol University
Bibliographic Citation
International Journal of Molecular Sciences. Vol.9, No.12 (2008), 2724-2744
Suggested Citation
Orapin Wongsawatkul, Supaluk Prachayasittikul, Chartchalerm Isarankura-Na-Ayudhya, Jutamaad Satayavivad, Somsak Ruchirawat, Virapong Prachayasittikul Vasorelaxant and Antioxidant Activities of Spilanthes acmella Murr. International Journal of Molecular Sciences. Vol.9, No.12 (2008), 2724-2744. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/2098
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Title
Vasorelaxant and Antioxidant Activities of Spilanthes acmella Murr
Abstract
This study reports the effect of Spilanthes acmella Murr. extracts on
phenylephrine-induced contraction of rat thoracic aorta as well as their antioxidant activity.
Results show that the extracts exert maximal vasorelaxations in a dose-dependent manner,
but their effects are less than acetylcholine-induced nitric oxide (NO) vasorelaxation.
Significant reduction of vasorelaxations is observed in both NG-nitro-L-arginine methyl
ester (L-NAME) and indomethacin (INDO). In the presence of L-NAME plus INDO,
synergistic effects are observed, leading to loss of vasorelaxation of both acetylcholine and
the extracts. Similarly, the vasorelaxations of the extracts are completely abolished upon
the removal of endothelial cells. This demonstrates that the extracts exhibit vasorelaxation via partially endothelium-induced NO and prostacyclin in a dose-dependent manner.
Significantly, the ethyl acetate extract exerts immediate vasorelaxation (ED50 76.1 ng/mL)
and is the most potent antioxidant (DPPH assay). The chloroform extract shows the highest
vasorelaxation and antioxidation (SOD assay). These reveal a potential source of
vasodilators and antioxidants.
