Publication: Partial biochemical characterisation of collagen in carious dentin of human primary teeth
Issued Date
2004-04-01
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ISSN
00039969
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2-s2.0-1542380483
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Mahidol University
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SCOPUS
Bibliographic Citation
Archives of Oral Biology. Vol.49, No.4 (2004), 267-273
Suggested Citation
S. Nakornchai, P. Atsawasuwan, E. Kitamura, R. Surarit, M. Yamauchi Partial biochemical characterisation of collagen in carious dentin of human primary teeth. Archives of Oral Biology. Vol.49, No.4 (2004), 267-273. doi:10.1016/j.archoralbio.2003.11.003 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/21207
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Title
Partial biochemical characterisation of collagen in carious dentin of human primary teeth
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Abstract
The process of dentin caries is a complex event involving demineralisation and matrix degradation. The objective of this study was to biochemically characterise collagen, the major dentin matrix component, by amino acid and cross-link analyses in two distinct carious dentin regions of human primary teeth. Twenty-seven carious primary teeth were obtained from 3 to 11-year-old patients and three layers of dentin, i.e. outer, inner carious dentin and normal dentin, were identified by a 1% acid red solution and dissected from each tooth. The samples were pulverised, the respective layers obtained from three to five teeth were pooled and six samples per layer were analysed. Aliquots of the dried dentin powder were hydrolysed with 6N HCl at 110°C for 24h and subjected to amino acid analyses. Other aliquots were demineralised, reduced with standardised NaB3H4, hydrolysed and subjected to quantitative collagen cross-link analyses. The results demonstrated that in the outer carious layer the collagen-associated amino acids were significantly lower and the reducible cross-links were markedly diminished when compared to the other two groups. There was no significant difference in these parameters between the inner carious and the normal layers. The data indicate that while the collagen in the outer carious dentin is significantly altered and degraded, the one in the inner carious dentin is relatively unaffected in primary teeth. © 2003 Elsevier Ltd. All rights reserved.