Publication: Sensitivity and specificity of a lateral flow immunoassay (LFI) in serum samples for diagnosis of melioidosis
Issued Date
2018-12-01
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ISSN
18783503
00359203
00359203
Other identifier(s)
2-s2.0-85057205970
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Mahidol University
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SCOPUS
Bibliographic Citation
Transactions of the Royal Society of Tropical Medicine and Hygiene. Vol.112, No.12 (2018), 568-570
Suggested Citation
Gumphol Wongsuvan, Viriya Hantrakun, Prapit Teparrukkul, Mallika Imwong, T. Eoin West, Vanaporn Wuthiekanun, Nicholas P.J. Day, David Aucoin, Direk Limmathurotsakul Sensitivity and specificity of a lateral flow immunoassay (LFI) in serum samples for diagnosis of melioidosis. Transactions of the Royal Society of Tropical Medicine and Hygiene. Vol.112, No.12 (2018), 568-570. doi:10.1093/trstmh/try099 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/45933
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Title
Sensitivity and specificity of a lateral flow immunoassay (LFI) in serum samples for diagnosis of melioidosis
Abstract
© 2018 The Author(s). Background Culture is the gold standard for the diagnosis of melioidosis, an infection caused by Burkholderia pseudomallei. Here we evaluate a lateral flow immunoassay (LFI) to detect B. pseudomallei capsular polysaccharide (CPS) in serum samples. Methods Patients with culture from any clinical specimen positive for B. pseudomallei were selected as cases. Patients who were blood culture positive for Staphylococcus aureus, Escherichia coli or Klebsiella pneumoniae as well as those who were malaria or dengue polymerase chain reaction assay positive were selected as controls. Results The sensitivity of the LFI was 31.3% (60/192 case patients [95% confidence interval {CI} 24.8 to 38.3]) and the specificity was 98.8% (559/566 control patients [95% CI 97.4 to 99.5]) in serum samples. Conclusions Although LFI may have limited sensitivity in serum, it can rapidly diagnose melioidosis in resource-limited settings.