Publication: Differential colony size, cell length, and cellular proteome of Escherichia coli isolated from urine vs. stone nidus of kidney stone patients
Issued Date
2017-03-01
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ISSN
18733492
00098981
00098981
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2-s2.0-85009901983
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Mahidol University
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SCOPUS
Bibliographic Citation
Clinica Chimica Acta. Vol.466, (2017), 112-119
Suggested Citation
Ratree Tavichakorntrakool, Patcharee Boonsiri, Vitoon Prasongwatana, Aroonlug Lulitanond, Chaisiri Wongkham, Visith Thongboonkerd Differential colony size, cell length, and cellular proteome of Escherichia coli isolated from urine vs. stone nidus of kidney stone patients. Clinica Chimica Acta. Vol.466, (2017), 112-119. doi:10.1016/j.cca.2016.12.018 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/41978
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Title
Differential colony size, cell length, and cellular proteome of Escherichia coli isolated from urine vs. stone nidus of kidney stone patients
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Abstract
© 2017 Elsevier B.V. Background Escherichia coli is associated with kidney stone disease, as a cause or an effect (secondary or recurrent urinary tract infection, UTI). Defining phenotypic or functional differences between E. coli inside stone nidus (ECS, associated with infection-induced stone) and outside the stone (i.e. from urine) (ECU, represented secondary infection) would be helpful to better understand bacterial involvement in this disease. Methods ECS and ECU were isolated from 100 stone formers and subjected to antimicrobial susceptibility test, ERIC-PCR genotyping, determination of biofilm formation, bacterial colony size on agar plate and cell length in broth, 2-DE, nanoLC-MS/MS, protein network analysis, and pyruvate dehydrogenase (PDH) activity assay. Results From 100 stone formers, 36 had positive bacterial culture, of which 5 pairs had identical antimicrobial susceptibility patterns and comparable ERIC-PCR genotypes. ECS had smaller colony size and longer cell length than ECU. 2-DE proteomic analysis revealed significantly differential levels of proteins involved in carbohydrate metabolism, stress response, and RNA/protein metabolism. Functional validation demonstrated lower PDH activity in ECS. Conclusions All these differential phenotypic and cellular proteome findings might be adaptive response of E. coli from remote infection to survive within the stone matrix that subsequently caused recurrent UTI in kidney stone patients.
