Publication: Comparison of UV spectrophotometric method and high performance liquid chromatography for the analysis of flunarizine and its application for the dissolution test
Issued Date
2006-04-11
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ISSN
07317085
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2-s2.0-33645080917
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Pharmaceutical and Biomedical Analysis. Vol.41, No.1 (2006), 158-164
Suggested Citation
Kesarin Busaranon, Worapot Suntornsuk, Leena Suntornsuk Comparison of UV spectrophotometric method and high performance liquid chromatography for the analysis of flunarizine and its application for the dissolution test. Journal of Pharmaceutical and Biomedical Analysis. Vol.41, No.1 (2006), 158-164. doi:10.1016/j.jpba.2005.11.008 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/23168
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Title
Comparison of UV spectrophotometric method and high performance liquid chromatography for the analysis of flunarizine and its application for the dissolution test
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Abstract
This study aimed to develop a simple UV spectrophotometric method for the analysis and the dissolution test of flunarizine in capsules. The UV absorbance was both measured directly and by the first derivative measurements at 254 and 268 nm, respectively. The developed methods were validated for their linearity, accuracy, precision, limit of detection (LOD) and limit of quantitation (LOQ) in comparison with the reported HPLC method. The UV spectrophotometric method illustrated excellent linearity (r2> 0.9999) in the concentration range of 6-24 μg/mL. Precision (%R.S.D. < 1.50) and recoveries were good (%R > 99.62). The LOD of direct UV and first derivative measurements were 0.09 and 0.84 μg/mL, respectively, and the LOQ were 0.26 and 2.55 μg/mL, respectively. Results from the assay of flunarizine in capsules by the UV spectrophotometric methods, both direct and first derivative measurements were not significantly different from those of the HPLC method (P > 0.05). Additionally, the method was successfully used for the dissolution test of flunarizine capsule and was found to be reliable, simple, fast, and inexpensive. © 2005 Elsevier B.V. All rights reserved.