Publication:
Cariogenic properties of Streptococcus mutans clinical isolates with sortase defects

dc.contributor.authorJinthana Lapirattanakulen_US
dc.contributor.authorYukiko Takashimaen_US
dc.contributor.authorPornpen Tantivitayakulen_US
dc.contributor.authorThaniya Maudcheingkaen_US
dc.contributor.authorPattarawadee Leelataweewuden_US
dc.contributor.authorKazuhiko Nakanoen_US
dc.contributor.authorMichiyo Matsumoto-Nakanoen_US
dc.contributor.otherMahidol Universityen_US
dc.contributor.otherOkayama Universityen_US
dc.contributor.otherOsaka Universityen_US
dc.date.accessioned2018-12-21T06:43:46Z
dc.date.accessioned2019-03-14T08:02:49Z
dc.date.available2018-12-21T06:43:46Z
dc.date.available2019-03-14T08:02:49Z
dc.date.issued2017-09-01en_US
dc.description.abstract© 2017 Elsevier Ltd Objective In Streptococcus mutans, a Gram-positive pathogen of dental caries, several surface proteins are anchored by the activity of sortase enzyme. Although various reports have shown that constructed S. mutans mutants deficient of sortase as well as laboratory reference strains with a sortase gene mutation have low cariogenic potential, no known studies have investigated clinical isolates with sortase defects. Here, we examined the cariogenic properties of S. mutans clinical isolates with sortase defects as well as caries status in humans harboring such defective isolates. Design Sortase-defective clinical isolates were evaluated for biofilm formation, sucrose-dependent adhesion, stress-induced dextran-dependent aggregation, acid production, and acid tolerance. Additionally, caries indices of subjects possessing such defective isolates were determined. Results Our in vitro results indicated that biofilm with a lower quantity was formed by sortase-defective as compared to non-defective isolates. Moreover, impairments of sucrose-dependent adhesion and stress-induced dextran-dependent aggregation were found among the isolates with defects, whereas no alterations were seen in regard to acid production or tolerance. Furthermore, glucan-binding protein C, a surface protein anchored by sortase activity, was predominantly detected in culture supernatants of all sortase-defective S. mutans isolates. Although the sortase-defective isolates showed lower cariogenic potential because of a reduction in some cariogenic properties, deft/DMFT indices revealed that all subjects harboring those isolates had caries experience. Conclusions Our findings suggest the impairment of cariogenic properties in S. mutans clinical isolates with sortase defects, though the detection of these defective isolates seemed not to imply low caries risk in the subjects harboring them.en_US
dc.identifier.citationArchives of Oral Biology. Vol.81, (2017), 7-14en_US
dc.identifier.doi10.1016/j.archoralbio.2017.04.018en_US
dc.identifier.issn18791506en_US
dc.identifier.issn00039969en_US
dc.identifier.other2-s2.0-85018308546en_US
dc.identifier.urihttps://repository.li.mahidol.ac.th/handle/20.500.14594/41810
dc.rightsMahidol Universityen_US
dc.rights.holderSCOPUSen_US
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85018308546&origin=inwarden_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.subjectDentistryen_US
dc.titleCariogenic properties of Streptococcus mutans clinical isolates with sortase defectsen_US
dc.typeArticleen_US
dspace.entity.typePublication
mu.datasource.scopushttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85018308546&origin=inwarden_US

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