Publication: DNA typing from fluorescent powder dusted latent fingerprints
Issued Date
2011-12-01
Resource Type
ISSN
1875175X
18751768
18751768
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2-s2.0-82455210963
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Mahidol University
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SCOPUS
Bibliographic Citation
Forensic Science International: Genetics Supplement Series. Vol.3, No.1 (2011)
Suggested Citation
Chatchadaporn Thamnurak, Wanasphon Bunakkharasawat, Suda Riengrojpitak, Nathinee Panvisavas DNA typing from fluorescent powder dusted latent fingerprints. Forensic Science International: Genetics Supplement Series. Vol.3, No.1 (2011). doi:10.1016/j.fsigss.2011.10.009 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/11408
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Title
DNA typing from fluorescent powder dusted latent fingerprints
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Abstract
DNA profiles were successfully typed from four latent fingerprints deposited on glass plate, glossy magazine paper, and plastic surface that were dusted with red, green, and yellow fluorescent powder. No or partial DNA profile was generated from one latent fingerprint samples. In addition, two DNA extraction methods, i.e., QIAamp ® DNA Mini Kit and Chelex ® 100 were compared. Experiments demonstrated that the absorbance measurements by spectrophotometery were interfered by colors of the fluorescent powder extracts, although these did not affect DNA profiling and detection of the PCR-amplified products by the genetic analyzer instrument. Results demonstrated that QIAamp ® DNA Mini Kit was more suitable than Chelex ® 100 for recovery of DNA from fluorescent powder dusted fingerprints. The quality of partial DNA profiles obtained from fluorescent powder dusted fingerprints was improved through the application of low copy number (LCN) typing, by increasing the number of PCR cycles from 28 to 34. Surface type had an effect on the number of loci obtained, and fluorescent fingerprint powders used had subtle effect on the profile quality obtained. © 2011 Elsevier Ireland Ltd.