Publication:
Synthesis of homo- and hetero-oligosaccharides by Thai rosewood β- glucosidase

Issued Date

1999-12-01

Resource Type

Article

ISSN

01415492

DOI

10.1023/A:1005626209655

Other identifier(s)

2-s2.0-0033403698

Rights

Mahidol University

Rights Holder(s)

SCOPUS

Bibliographic Citation

Biotechnology Letters. Vol.21, No.11 (1999), 947-951

Suggested Citation

Chantragan Srisomsap, Pantipa Subhasitanont, Supanna Techasakul, Rudee Surarit, Jisnuson Svasti Synthesis of homo- and hetero-oligosaccharides by Thai rosewood β- glucosidase. Biotechnology Letters. Vol.21, No.11 (1999), 947-951. doi:10.1023/A:1005626209655 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/25324

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Title

Synthesis of homo- and hetero-oligosaccharides by Thai rosewood β- glucosidase

Author(s)

Chantragan Srisomsap
 Pantipa Subhasitanont
 Supanna Techasakul
 Rudee Surarit
 Jisnuson Svasti

Other Contributor(s)

Chulabhorn Research Institute
 Mahidol University

Abstract

β-Glucosidase from Thai Rosewood (Dalbergia cochinchinensis Pierre) catalyzes the synthesis disaccharides and trisaccharides at 40-70% (w/w) glucose. Gentiobiose (β1-6-diglucose) was the major disaccharide. The reaction was optimal at pH 5.0 and 60-65°C. When the reaction was performed in mixtures of glucose and fucose, a novel product was found, which was purified and characterized by NMR spectroscopy to be D-fucosyl (β1-6)-D- glucose.

Keyword(s)

Biochemistry, Genetics and Molecular Biology
 Chemical Engineering
 Immunology and Microbiology

Availability

URI

https://repository.li.mahidol.ac.th/handle/20.500.14594/25324

Collections

Scopus 1991-2000