Publication: The Burkholderia pseudomallei oxyR gene: Expression analysis and mutant characterization
Issued Date
2002-08-21
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ISSN
03781119
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2-s2.0-0037151774
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Mahidol University
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SCOPUS
Bibliographic Citation
Gene. Vol.296, No.1-2 (2002), 161-169
Suggested Citation
Suvit Loprasert, Ratiboot Sallabhan, Wirongrong Whangsuk, Skorn Mongkolsuk The Burkholderia pseudomallei oxyR gene: Expression analysis and mutant characterization. Gene. Vol.296, No.1-2 (2002), 161-169. doi:10.1016/S0378-1119(02)00854-5 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/20050
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Title
The Burkholderia pseudomallei oxyR gene: Expression analysis and mutant characterization
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Abstract
Burkholderia pseudomallei (Bp) is the causative agent of the life-threatening melioidosis in humans. The global transcription factor oxyR gene was isolated and characterized. It is located between recG, encoding a putative DNA helicase, and katG, encoding a putative catalase-peroxidase. oxyR is expressed as a monocistronic 1 kb mRNA and is induced by oxidative stress compounds. Northern, primer extension, and transcription reporter fusion analyses showed that oxyR mRNA is induced by 0.2 mM menadione, 2 mM paraquat, and 10 mM H2O2. Two knockout mutants of oxyR were constructed, by single- and double-crossover recombination, and found to be hypersensitive to H2O2and paraquat. Bp lacking OxyR exhibited autoaggregation when cultured in liquid broth and an increased ability to form biofilms in minimal medium, but not in Luria-Bertani broth. The oxyR mutants also have a decreased level of extracellular protease activity. The altered phenotypes of oxyR deficient mutants were complemented when a copy of oxyR was transposed into the mutant chromosomes on the mini-Tn5 transposon. © 2002 Elsevier Science B.V. All rights reserved.