Publication: Human kidney anion exchanger 1 interacts with kinesin family member 3B (KIF3B)
Issued Date
2011-09-16
Resource Type
ISSN
10902104
0006291X
0006291X
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2-s2.0-80052833884
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Mahidol University
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SCOPUS
Bibliographic Citation
Biochemical and Biophysical Research Communications. Vol.413, No.1 (2011), 69-74
Suggested Citation
Natapol Duangtum, Mutita Junking, Nunghathai Sawasdee, Boonyarit Cheunsuchon, Thawornchai Limjindaporn, Pa thai Yenchitsomanus Human kidney anion exchanger 1 interacts with kinesin family member 3B (KIF3B). Biochemical and Biophysical Research Communications. Vol.413, No.1 (2011), 69-74. doi:10.1016/j.bbrc.2011.08.050 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/11471
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Title
Human kidney anion exchanger 1 interacts with kinesin family member 3B (KIF3B)
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Abstract
Impaired trafficking of human kidney anion exchanger 1 (kAE1) to the basolateral membrane of α-intercalated cells of the kidney collecting duct leads to the defect of the Cl - /HCO3- exchange and the failure of proton (H + ) secretion at the apical membrane of these cells, causing distal renal tubular acidosis (dRTA). In the sorting process, kAE1 interacts with AP-1 mu1A, a subunit of AP-1A adaptor complex. However, it is not known whether kAE1 interacts with motor proteins in its trafficking process to the plasma membrane or not. We report here that kAE1 interacts with kinesin family member 3B (KIF3B) in kidney cells and a dileucine motif at the carboxyl terminus of kAE1 contributes to this interaction. We have also demonstrated that kAE1 co-localizes with KIF3B in human kidney tissues and the suppression of endogenous KIF3B in HEK293T cells by small interfering RNA (siRNA) decreases membrane localization of kAE1 but increases its intracellular accumulation. All results suggest that KIF3B is involved in the trafficking of kAE1 to the plasma membrane of human kidney α-intercalated cells. © 2011 Elsevier Inc.