Publication: Ultrasensitive detection of lung cancer-associated miRNAs by multiple primer-mediated rolling circle amplification coupled with a graphene oxide fluorescence-based (MPRCA-GO) sensor
Issued Date
2019-07-21
Resource Type
ISSN
13645528
00032654
00032654
Other identifier(s)
2-s2.0-85068605752
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Mahidol University
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SCOPUS
Bibliographic Citation
Analyst. Vol.144, No.14 (2019), 4180-4187
Suggested Citation
Krissana Khoothiam, Kiatnida Treerattrakoon, Tawin Iempridee, Patraporn Luksirikul, Tararaj Dharakul, Deanpen Japrung Ultrasensitive detection of lung cancer-associated miRNAs by multiple primer-mediated rolling circle amplification coupled with a graphene oxide fluorescence-based (MPRCA-GO) sensor. Analyst. Vol.144, No.14 (2019), 4180-4187. doi:10.1039/c9an00517j Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/50123
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Title
Ultrasensitive detection of lung cancer-associated miRNAs by multiple primer-mediated rolling circle amplification coupled with a graphene oxide fluorescence-based (MPRCA-GO) sensor
Abstract
© 2019 The Royal Society of Chemistry. MicroRNAs (miRNAs) play important roles in gene regulation and have been reported as biomarkers in cancer diagnosis. Herein, we develop an isothermal miRNA detection platform based on the highly efficient, multiple primer-mediated rolling circle amplification method coupled with a graphene oxide-based fluorescence (MPRCA-GO) assay, using lung cancer-associated miRNAs (miR-21 and miR-210) and a reference miRNA (miR-16) as model targets. The combination of the designed ssDNA probe and T4 RNA ligase (T4 Rnl2) used in the MPRCA-GO assay allowed for single-base mismatch discrimination. In addition, the superfluorescence quenching ability of GO allowed for rapid fluorescence detection. The developed platform had a limit of detection as low as 0.87 fM and could detect target miRNAs in cancer cell lines and human serums. Therefore, the MPRCA-GO sensor has the potential for single nucleotide polymorphism (SNP) analysis and applications in clinical diagnostics.