Publication: SCAR markers and multiplex PCR-based identification of isomorphic species in the Anopheles dirus complex in Southeast Asia
2
Issued Date
2002-01-01
Resource Type
ISSN
0269283X
Other identifier(s)
2-s2.0-0036010084
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Mahidol University
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SCOPUS
Bibliographic Citation
Medical and Veterinary Entomology. Vol.16, No.1 (2002), 46-54
Suggested Citation
Sylvie Manguin, P. Kengne, L. Sonnier, R. E. Harbach, V. Baimai, H. D. Trung, M. Coosemans SCAR markers and multiplex PCR-based identification of isomorphic species in the Anopheles dirus complex in Southeast Asia. Medical and Veterinary Entomology. Vol.16, No.1 (2002), 46-54. doi:10.1046/j.0269-283x.2002.00344.x Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/20013
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Title
SCAR markers and multiplex PCR-based identification of isomorphic species in the Anopheles dirus complex in Southeast Asia
Other Contributor(s)
Centre de Biologie pour la Gestion des Populations
IRD Institut de Recherche pour le Developpement
CNRS Centre National de la Recherche Scientifique
The Natural History Museum, London
Mahidol University
National Institute of Malariology, Parasitology and Entomology Hanoi
Prins Leopold Instituut voor Tropische Geneeskunde
IRD Institut de Recherche pour le Developpement
CNRS Centre National de la Recherche Scientifique
The Natural History Museum, London
Mahidol University
National Institute of Malariology, Parasitology and Entomology Hanoi
Prins Leopold Instituut voor Tropische Geneeskunde
Abstract
The Anopheles dirus Peyton & Harrison complex of mosquitoes (Diptera: Culicidae) comprises seven known species, including important malaria vectors in Southeast Asia. Specific identification of each species of the complex, which cannot be distinguished using morphological characters, is crucial for understanding vector ecology and implementing effective control measures. Derived from individual random amplified polymorphic DNA (RAPD) markers, sequence characterized amplified regions (SCAR) were analysed for the design of specific paired-primers. Combination of six SCAR primers resulted in the development of a simple, robust, single multiplex PCR able to identify three important malaria vectors among the four most common species (A, B, C, D) of the complex: species A from several Southeast Asian countries, species B from Perlis, Malaysia, and species C and D from Thailand.