Publication: Simultaneous determination of trans,trans-muconic acid and S-phenylmercapturic acid by high pressure liquid chromatography and its application
Issued Date
2004-09-01
Resource Type
ISSN
01251562
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2-s2.0-8444236752
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Mahidol University
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SCOPUS
Bibliographic Citation
Southeast Asian Journal of Tropical Medicine and Public Health. Vol.35, No.3 (2004), 717-723
Suggested Citation
Prapin Tharnpoophasiam, Pornpimol Kongtip, Waranya Wongwit, Wijitr Fungladda, Dwip Kitayaporn Simultaneous determination of trans,trans-muconic acid and S-phenylmercapturic acid by high pressure liquid chromatography and its application. Southeast Asian Journal of Tropical Medicine and Public Health. Vol.35, No.3 (2004), 717-723. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/21540
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Title
Simultaneous determination of trans,trans-muconic acid and S-phenylmercapturic acid by high pressure liquid chromatography and its application
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Abstract
The simultaneous determination of urinary trans,trans-muconic acid (t,t-MA) and S-phenylmercapturic acid (S-PMA) was performed by liquid extraction with ethyl acetate and reversed-phase high performance liquid chromatography (RP-HPLC) on a Hypersil-ODS column using the gradient mobile phase of methanol and 0.0012 N perchloric acid and diode array detection at 205 and 264 nm for S-PMA and t,t-MA, respectively. The retention times for t,t-MA and S-PMA were 3.8 and 12.3 minutes, respectively. The recoveries of t,t-MA and S-PMA were > 97%; between-day precisions were all within 8% RSD (100x SD/mean). The method was applied to analyze the urinary t,t-MA and S-PMA of 59 service station attendants exposed to average benzene concentrations in the air of 0.20±0.18 ppm. Significant differences in pre-shift and post-shift urinary t,t-MA between smokers and non-smokers were found.