Publication: Genetic speciation of environmental Legionella isolates in Thailand
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Issued Date
2012-10-01
Resource Type
ISSN
15677257
15671348
15671348
Other identifier(s)
2-s2.0-84865391109
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Infection, Genetics and Evolution. Vol.12, No.7 (2012), 1368-1376
Suggested Citation
Wantana Paveenkittiporn, Surang Dejsirilert, Thareerat Kalambaheti Genetic speciation of environmental Legionella isolates in Thailand. Infection, Genetics and Evolution. Vol.12, No.7 (2012), 1368-1376. doi:10.1016/j.meegid.2012.03.025 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/13390
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Title
Genetic speciation of environmental Legionella isolates in Thailand
Other Contributor(s)
Abstract
Legionella-like organisms were isolated during 2003-2007 from various water resources by culturing on selective media of Wadowsky-Yee-Okuda agar. The 256 isolates were identified as belonging to the Legionella genus based on detection of 108. bp PCR product of the 5S rRNA gene, while the inclusion as Legionella pneumophila were confirmed by PCR detection of a specific mip gene region of 168. bp. The 50 isolates, identified as non-pneumophila, were then subjected to DNA tree analysis, based on mip gene of ∼650. bp and rnpB genes product ranged from 304 to 354. bp. Phylogenetic tree was constructed to predict their species in relative to the available database. The isolates of which their speciation, based on those two genes were inconclusive, were then investigated for the almost full-length of 16S rRNA sequences. The isolates were assigned as 16 known Legionella species, and proposed seven novel species based on their unique 16S rRNA sequence. © 2012 Elsevier B.V.