Publication: Synthesis and in vitro transfection efficiency of spermine-based cationic lipids with different central core structures and lipophilic tails
Issued Date
2015-02-01
Resource Type
ISSN
14643405
0960894X
0960894X
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2-s2.0-84920767671
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Mahidol University
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SCOPUS
Bibliographic Citation
Bioorganic and Medicinal Chemistry Letters. Vol.25, No.3 (2015), 496-503
Suggested Citation
Nattisa Niyomtham, Nuttapon Apiratikul, Kanoknetr Suksen, Praneet Opanasopit, Boon Ek Yingyongnarongkul Synthesis and in vitro transfection efficiency of spermine-based cationic lipids with different central core structures and lipophilic tails. Bioorganic and Medicinal Chemistry Letters. Vol.25, No.3 (2015), 496-503. doi:10.1016/j.bmcl.2014.12.043 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/35504
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Title
Synthesis and in vitro transfection efficiency of spermine-based cationic lipids with different central core structures and lipophilic tails
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Abstract
© 2015 Published by Elsevier Ltd. Twelve spermine-based cationic lipids with four different central core structures (di(oxyethyl)amino, di(oxyethyl)amino carboxy, 3-amino-1,2-dioxypropyl and 2-amino-1,3-dioxypropyl) and three hydrophobic tails (lauric acid, myristic acid and palmitic acid) were synthesized. The liposomes containing lipids and DOPE showed moderate to good in vitro DNA delivery into HeLa cells. GFP expression experiments revealed that liposomes composed of lipids with 3-amino-1,2-dioxypropyl as a central core structure exhibited highest transfection efficiency under serum-free condition. Whereas, lipid with 2-amino-1,3-dioxypropyl core structure showed highest transfection under 10% serum condition. Moreover, the liposomes and lipoplexes composted of these cationic lipids exhibited low cytotoxicity.