Publication: Bioactive metabolites from spilanthes acmella murr
7
1
Issued Date
2009
Resource Type
Language
eng
ISSN
1420-3049
Rights
Mahidol University
Bibliographic Citation
Molecules. Vol.14, No.2 (2009), 850-867
Suggested Citation
Supaluk Prachayasittikul, Saowapa Suphapong, Apilak Worachartcheewan, Ratana Lawung, Somsak Ruchirawat, Virapong Prachayasittikul Bioactive metabolites from spilanthes acmella murr. Molecules. Vol.14, No.2 (2009), 850-867. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/2111
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Bioactive metabolites from spilanthes acmella murr
Abstract
Spilanthes acmella Murr. (Compositae) has been used as a traditional medicine
for toothache, rheumatism and fever. Its extracts had been shown to exhibit vasorelaxant
and antioxidant activities. Herein, its antimicrobial, antioxidant and cytotoxic activities
were evaluated. Agar dilution method assays against 27 strains of microorganisms were
performed. Results showed that fractions from the chloroform and methanol extracts
inhibited the growth of many tested organisms, e.g. Corynebacterium diphtheriae NCTC
10356 with minimum inhibitory concentration (MIC) of 64-256 μg/mL and Bacillus
subtilis ATCC 6633 with MIC of 128-256 μg/mL. The tested fractions all exhibited
antioxidant properties in both DPPH and SOD assays. Potent radical scavenging activity
was observed in the DPPH assay. No cytotoxic effects of the extracts against KB and
HuCCA-1 cell lines were evident. Bioassay-guided isolation resulted in a diverse group of
bioactive compounds such as phenolics [vanillic acid (2), trans-ferulic acid (5) and transisoferulic
acid (6)], coumarin (scopoletin, 4) and triterpenoids like 3-acetylaleuritolic acid
(1), β-sitostenone (3), stigmasterol and stigmasteryl-3-O- β-D-glucopyranosides, in
addition to a mixture of stigmasteryl-and β-sitosteryl-3-O- β-D-glucopyranosides. The
compounds 1–6 represent bioactive metabolites of S. acmella Murr. that were never previously reported. Our findings demonstrate for the first time the potential benefits of
this medicinal plant as a rich source of high therapeutic value compounds for medicines,
cosmetics, supplements and as a health food.