Publication: Lipopolysaccharides from different Burkholderia species with different lipid a structures induce toll-like receptor 4 activation and react with melioidosis patient sera
Issued Date
2019-12-01
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ISSN
10985522
00199567
00199567
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2-s2.0-85075222273
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Mahidol University
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SCOPUS
Bibliographic Citation
Infection and Immunity. Vol.87, No.12 (2019)
Suggested Citation
Sineenart Sengyee, Sung Hwan Yoon, T. Eoin West, Robert K. Ernst, Narisara Chantratita Lipopolysaccharides from different Burkholderia species with different lipid a structures induce toll-like receptor 4 activation and react with melioidosis patient sera. Infection and Immunity. Vol.87, No.12 (2019). doi:10.1128/IAI.00692-19 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/50987
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Title
Lipopolysaccharides from different Burkholderia species with different lipid a structures induce toll-like receptor 4 activation and react with melioidosis patient sera
Abstract
Copyright © 2019 Sengyee et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license. Lipopolysaccharides (LPSs) of Gram-negative bacteria comprise lipid A, core, and O-polysaccharide (OPS) components. Studies have demonstrated that LPSs isolated from the pathogenic species Burkholderia pseudomallei and Burkholderia mallei and from less-pathogenic species, such as Burkholderia thailandensis, are potent immune stimulators. The LPS structure of B. pseudomallei, the causative agent of melioidosis, is highly conserved in isolates from Thailand; however, the LPSs isolated from other, related species have not been characterized to enable understanding of their immune recognition and antigenicities. Here, we describe the structural and immunological characteristics of the LPSs isolated from eight Burkholderia species and compare those for B. pseudomallei to those for the other seven species. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), gas chromatography (GC), SDS-PAGE, Toll-like receptor 4 (TLR4) stimulation, and immunoblot analysis were performed on these Burkholderia species. MALDI-TOF profiles demonstrated that Burkholderia lipid A contains predominantly penta-acylated species modified with 4-amino-4-deoxy-arabinose residues at both terminal phosphate groups. The lipid A could be differentiated based on mass differences at m/z 1,511, 1,642, 1,773, and 1,926 and on fatty acid composition. LPSs of all species induced TLR4-dependent NF-κB responses; however, while SDS-PAGE analysis showed similar LPS ladder patterns for B. pseudomallei, B. thailandensis, and B. mallei, these patterns differed from those of other Burkholderia species. Interestingly, immunoblot analysis demonstrated that melioidosis patient sera cross-reacted with OPSs of other Burkholderia species. These findings can be used to better understand the characteristics of LPS in Burkholderia species, and they have implications for serological diagnostics based on the detection of antibodies to OPS.