Publication: Simultaneous detection and subtyping of H274Y-positive influenza a (H1N1) using pyrosequencing
Issued Date
2011-05-01
Resource Type
ISSN
19722680
20366590
20366590
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2-s2.0-79958103399
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Infection in Developing Countries. Vol.5, No.5 (2011), 348-352
Suggested Citation
Wasun Chantratita, Chonlaphat Sukasem, Sayomporn Sirinavin, Nipaporn Sankuntaw, Chutatip Srichantaratsamee, Ekawat Pasomsub, Kumthorn Malathum Simultaneous detection and subtyping of H274Y-positive influenza a (H1N1) using pyrosequencing. Journal of Infection in Developing Countries. Vol.5, No.5 (2011), 348-352. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/12052
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Title
Simultaneous detection and subtyping of H274Y-positive influenza a (H1N1) using pyrosequencing
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Abstract
Introduction: We investigated the frequency of H274Y-positive swine-origin 2009 A (H1N1) influenza virus outbreak in Thailand during May-August 2009. Methodology: This study sought to find Oseltamivir resistance mutation H274Y by using pyrosequencing. Results: From 8,710 real-time RT-PCR swine-origin 2009 A(H1N1) influenza virus-positive specimens, 100 randomly selected samples identified one such virus with H274Y mutation using pyrosequencing. Conclusions: The patient probably acquired oseltamivir resistance from natural variation, since he had never received that form of treatment before and recovered from influenza-like symptoms without using anti-influenza drugs. © 2011 Chantratita et al.