Publication: Opisthorchis viverrini: The effects of colchicine and cytochalasin B on the adult tegument
Issued Date
1996-06-01
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ISSN
01251562
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2-s2.0-0030154651
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Mahidol University
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SCOPUS
Bibliographic Citation
Southeast Asian Journal of Tropical Medicine and Public Health. Vol.27, No.2 (1996), 312-318
Suggested Citation
Prasert Sobhon, Wandee Apinhasmit Opisthorchis viverrini: The effects of colchicine and cytochalasin B on the adult tegument. Southeast Asian Journal of Tropical Medicine and Public Health. Vol.27, No.2 (1996), 312-318. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/17733
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Title
Opisthorchis viverrini: The effects of colchicine and cytochalasin B on the adult tegument
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Abstract
The roles of the tegumental cytoskeleton were tested by treating adult flukes with colchicine and cytochalasin B. Following a short incubation period (10-20 minutes), colchicine disrupted microtubules in the tegumental cells' processes which, in turn, affected the transport of dense granules from the cells' soma to the tegument; as a result some of these granules were fused together to form membrane-bound vacuoles. In addition, at many spots microtrabeculae were also depolymerized, which resulted in the formation of non-membrane-bound vacuoles and the distension of microvilli to form blebs, some of which were disrupted. After prolonged incubation (120 minutes), general breakdown of the tegumental cytoskeleton occurred, and parts of it were sloughed off. In cytochalasin B treatment, the responses were similar to those of colchicine but with less severity. After a short incubation period (10-20 minutes), the microtrabeculae were depolymerized which led to the formation of non-membrane-bound vacuoles in the apical and middle zones of the tegument. Later, the tegumental microvilli were distended to form blebs but no evidence of tegumental sloughing occurred even in prolonged incubation. From these obsevations, it was concluded that microtubules played a role in the translocation of granules from the tegumental cells to the tegument which modulated the synthesis of membrane and glycocalyx, while microtrabeculae were involved in the maintenance of the structure and integrity of the tegument.