Publication: Studies on immunodiagnosis of human paragonimiasis and specific antigen of Paragonimus heterotremus
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Issued Date
1991-01-01
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ISSN
00207519
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2-s2.0-0025776314
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Mahidol University
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SCOPUS
Bibliographic Citation
International Journal for Parasitology. Vol.21, No.4 (1991), 395-401
Suggested Citation
Isna Indrawati, Wanpen Chaicumpa, Prasert Setasuban, Yuwaporn Ruangkunaporn Studies on immunodiagnosis of human paragonimiasis and specific antigen of Paragonimus heterotremus. International Journal for Parasitology. Vol.21, No.4 (1991), 395-401. doi:10.1016/0020-7519(91)90096-P Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/22051
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Title
Studies on immunodiagnosis of human paragonimiasis and specific antigen of Paragonimus heterotremus
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Abstract
Adult Paragonimus heterotremus were recovered from the lungs and pleural cavity of cats orally infected with metacercariae. The worms were ground and extracted with distilled water. The soluble crude antigen (CA) contained about 40% proteins which could be fractionated by gel filtration on Sephadex G-200 into three profiles namely the F1, F2and F3. The CA and its Sephadex profiles were used in an indirect enzyme-linked immunosorbent assay (ELISA) for detecting antibodies to P. heterotemus in three groups of patients, i.e. patients whose sputum and/or faeces revealed P. heterotremus eggs (group 1), patients with other parasitic infections (group 2), bacterial proven tuberculosis patients (group 3) and healthy, parasite-free controls (group 4). The sensitivity and specificity of the assay when the F1was used as the antigen were 100%. Western blot analysis revealed that specific antigen of P. heterotremus was a non-protein component of Mr35 kDa. © 1991.