Publication: Antibody-conjugated paramagnetic nanobeads: Kinetics of bead-cell binding
Issued Date
2014-05-19
Resource Type
ISSN
14220067
16616596
16616596
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2-s2.0-84901020289
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Mahidol University
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SCOPUS
Bibliographic Citation
International Journal of Molecular Sciences. Vol.15, No.5 (2014), 8821-8834
Suggested Citation
Shahid Waseem, Michael A. Allen, Stefan Schreier, Rachanee Udomsangpetch, Sebastian C. Bhakdi Antibody-conjugated paramagnetic nanobeads: Kinetics of bead-cell binding. International Journal of Molecular Sciences. Vol.15, No.5 (2014), 8821-8834. doi:10.3390/ijms15058821 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/33261
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Title
Antibody-conjugated paramagnetic nanobeads: Kinetics of bead-cell binding
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Abstract
Specific labelling of target cell surfaces using antibody-conjugated paramagnetic nanobeads is essential for efficient magnetic cell separation. However, studies examining parameters determining the kinetics of bead-cell binding are scarce. The present study determines the binding rates for specific and unspecific binding of 150 nm paramagnetic nanobeads to highly purified target and non-target cells. Beads bound to cells were enumerated spectrophotometrically. Results show that the initial bead-cell binding rate and saturation levels depend on initial bead concentration and fit curves of the form A(1 - exp(-kt)). Unspecific binding within conventional experimental time-spans (up to 60 min) was not detectable photometrically. For CD3-positive cells, the probability of specific binding was found to be around 80 times larger than that of unspecific binding. © 2014 by the authors; licensee MDPI, Basel, Switzerland.