Publication: Comparison of free radical scavenging activity of Siamese neem tree (Azadirachta indica A. Juss var. siamensis Valeton) leaf extracts prepared by different methods of extraction
Issued Date
2006-04-01
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10117571
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2-s2.0-33646464111
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Mahidol University
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SCOPUS
Bibliographic Citation
Medical Principles and Practice. Vol.15, No.3 (2006), 219-222
Suggested Citation
Pongtip Sithisarn, Roongtawan Supabphol, Wandee Gritsanapan Comparison of free radical scavenging activity of Siamese neem tree (Azadirachta indica A. Juss var. siamensis Valeton) leaf extracts prepared by different methods of extraction. Medical Principles and Practice. Vol.15, No.3 (2006), 219-222. doi:10.1159/000092185 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/23782
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Title
Comparison of free radical scavenging activity of Siamese neem tree (Azadirachta indica A. Juss var. siamensis Valeton) leaf extracts prepared by different methods of extraction
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Abstract
Objective: The aim of this study was to investigate the antioxidant activity of the aqueous extracts of leaves of Siamese neem tree (Azadirachta indica A. Juss var. siamensis Valeton) from several extracting and drying methods using 2,2-diphenyl-1-picrylhydrazyl (DPPH)-scavenging assay. Materials and Methods: The leaves of Siamese neem tree were extracted using percolation, decoction, maceration, soxhlet extraction, freeze drying or spray drying methods. The extract was tested for antioxidant activity using DPPH-scavenging assay. Thin-layer chromatography of the extract from decoction was also investigated. Results: The freeze drying method gave the highest yield (51.50%, w/w) of crude extract, while decoction gave the most effective DPPH-scavenging activity (EC50: 31.4 μg/ml). Thin-layer chromatography analysis was used to screen the leaf extract obtained using decoction, and the chromatogram showed spots corresponding to quercetin and rutin flavonoids which exhibited antioxidant activities (EC50: 2.29 and 34.67 μg/ml, respectively). Conclusion: Siamese neem tree leaf extracts possessed free radical scavenging activity against the DPPH radical. The most active extract was obtained with the leaf decoction method. It showed antioxidant activity with EC50 of 31.4 μg/ml. Copyright © 2006 S. Karger AG.