Publication: Melioidosis ds rapid test: A standardized serological dipstick assay with increased sensitivity and reliability due to multiplex detection
Issued Date
2020-07-01
Resource Type
ISSN
19352735
19352727
19352727
Other identifier(s)
2-s2.0-85089121037
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
PLoS Neglected Tropical Diseases. Vol.14, No.7 (2020), 1-16
Suggested Citation
Gabriel E. Wagner, Esther Föderl-Höbenreich, Karoline Assig, Michaela Lipp, Andreas Berner, Christian Kohler, Sabine Lichtenegger, Julia Stiehler, Wisansanee Karoonboonyanan, Nida Thanapattarapairoj, Chidchanok Promkong, Sirikamon Koosakulnirand, Panjaporn Chaichana, Ralf Ehricht, Annemarie Gad, Hans H. Söffing, Susanna J. Dunachie, Narisara Chantratita, Ivo Steinmetz Melioidosis ds rapid test: A standardized serological dipstick assay with increased sensitivity and reliability due to multiplex detection. PLoS Neglected Tropical Diseases. Vol.14, No.7 (2020), 1-16. doi:10.1371/journal.pntd.0008452 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/58106
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Melioidosis ds rapid test: A standardized serological dipstick assay with increased sensitivity and reliability due to multiplex detection
Author(s)
Gabriel E. Wagner
Esther Föderl-Höbenreich
Karoline Assig
Michaela Lipp
Andreas Berner
Christian Kohler
Sabine Lichtenegger
Julia Stiehler
Wisansanee Karoonboonyanan
Nida Thanapattarapairoj
Chidchanok Promkong
Sirikamon Koosakulnirand
Panjaporn Chaichana
Ralf Ehricht
Annemarie Gad
Hans H. Söffing
Susanna J. Dunachie
Narisara Chantratita
Ivo Steinmetz
Esther Föderl-Höbenreich
Karoline Assig
Michaela Lipp
Andreas Berner
Christian Kohler
Sabine Lichtenegger
Julia Stiehler
Wisansanee Karoonboonyanan
Nida Thanapattarapairoj
Chidchanok Promkong
Sirikamon Koosakulnirand
Panjaporn Chaichana
Ralf Ehricht
Annemarie Gad
Hans H. Söffing
Susanna J. Dunachie
Narisara Chantratita
Ivo Steinmetz
Other Contributor(s)
Institut für Photonische Technologien
Friedrich-Loeffler-Institute
Udon Thani Center Hospital
Friedrich Schiller Universität Jena
Khon Kaen Regional Hospital
Mahidol University
Nuffield Department of Medicine
Medizinische Universität Graz
Senova Gesellschaft für Biowissenschaft und Technik mbH
Nakhon Phanom Hospital
Friedrich-Loeffler-Institute
Udon Thani Center Hospital
Friedrich Schiller Universität Jena
Khon Kaen Regional Hospital
Mahidol University
Nuffield Department of Medicine
Medizinische Universität Graz
Senova Gesellschaft für Biowissenschaft und Technik mbH
Nakhon Phanom Hospital
Abstract
© 2020 Wagner et al. Background Melioidosis, caused by Burkholderia pseudomallei, is a severe infectious disease with high mortality rates, but is under-recognized worldwide. In endemic areas, there is a great need for simple, low-cost and rapid diagnostic tools. In a previous study we showed, that a protein multiplex array with 20 B. pseudomallei-specific antigens detects antibodies in melioidosis patients with high sensitivity and specificity. In a subsequent study the high potential of antiB. pseudomallei antibody detection was confirmed using a rapid Hcp1 single protein-based assay. Our protein array also showed that the antibody profile varies between patients, possibly due to a combination of host factors but also antigen variations in the infecting B. pseudomallei strains. The aim of this study was to develop a rapid test, combining Hcp1 and the best performing antigens BPSL2096, BPSL2697 and BPSS0477 from our previous study, to take advantage of simultaneous antibody detection. Methods and principal findings The 4-plex dipstick was validated with sera from 75 patients on admission plus control groups, achieving 92% sensitivity and 97–100% specificity. We then re-evaluated melioidosis sera with the 4-plex assay that were previously misclassified by the monoplex Hcp1 rapid test. 12 out of 55 (21.8%) false-negative samples were positive in our new dipstick assay. Among those, 4 sera (7.3%) were Hcp1 positive, whereas 8 (14.5%) sera remained Hcp1 negative but gave a positive reaction with our additional antigens. Conclusions Our dipstick rapid test represents an inexpensive, standardized and simple diagnostic tool with an improved serodiagnostic performance due to multiplex detection. Each additional band on the test strip makes a false-positive result more unlikely, contributing to its reliability. Future prospective studies will seek to validate the gain in sensitivity and specificity of our multiplex rapid test approach in different melioidosis patient cohorts.