Publication: Effect of Cell Seeding Density and Inflammatory Cytokines on Adipose Tissue-Derived Stem Cells: an in Vitro Study
Issued Date
2017-04-01
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ISSN
15586804
15508943
15508943
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2-s2.0-85010762625
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Mahidol University
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SCOPUS
Bibliographic Citation
Stem Cell Reviews and Reports. Vol.13, No.2 (2017), 267-277
Suggested Citation
Panithi Sukho, Jolle Kirpensteijn, Jan Willem Hesselink, Gerjo J.V.M. van Osch, Femke Verseijden, Yvonne M. Bastiaansen-Jenniskens Effect of Cell Seeding Density and Inflammatory Cytokines on Adipose Tissue-Derived Stem Cells: an in Vitro Study. Stem Cell Reviews and Reports. Vol.13, No.2 (2017), 267-277. doi:10.1007/s12015-017-9719-3 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/41908
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Title
Effect of Cell Seeding Density and Inflammatory Cytokines on Adipose Tissue-Derived Stem Cells: an in Vitro Study
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Abstract
© 2017, The Author(s). Adipose tissue-derived stem cells (ASCs) are known to be able to promote repair of injured tissue via paracrine factors. However, the effect of cell density and inflammatory cytokines on the paracrine ability of ASCs remains largely unknown. To investigate these effects, ASCs were cultured in 8000 cells/cm2, 20,000 cells/cm2, 50,000 cells/cm2, and 400,000 cells/cm2with and without 10 or 20 ng/ml tumor necrosis factor alpha (TNFα) and 25 or 50 ng/ml interferon gamma (IFNγ). ASC-sheets formed at 400,000 cells/cm2after 48 h of culture. With increasing concentrations of TNFα and IFNγ, ASC-sheets with 400,000 cells/cm2had increased production of angiogenic factors Vascular Endothelial Growth Factor and Fibroblast Growth Factor and decreased expression of pro-inflammatory genes TNFA and Prostaglandin Synthase 2 (PTGS2) compared to lower density ASCs. Moreover, the conditioned medium of ASC-sheets with 400,000 cells/cm2stimulated with the low concentration of TNFα and IFNγ enhanced endothelial cell proliferation and fibroblast migration. These results suggest that a high cell density enhances ASC paracrine function might beneficial for wound repair, especially in pro-inflammatory conditions.