Publication: Acute dengue virus 2 infection in Gabonese patients is associated with an early innate immune response, including strong interferon alpha production
Issued Date
2010-12-17
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ISSN
14712334
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2-s2.0-78650087190
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Mahidol University
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SCOPUS
Bibliographic Citation
BMC Infectious Diseases. Vol.10, (2010)
Suggested Citation
Pierre Becquart, Nadia Wauquier, Dieudonné Nkoghe, Angélique Ndjoyi-Mbiguino, Cindy Padilla, Marc Souris, Eric M. Leroy Acute dengue virus 2 infection in Gabonese patients is associated with an early innate immune response, including strong interferon alpha production. BMC Infectious Diseases. Vol.10, (2010). doi:10.1186/1471-2334-10-356 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/29343
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Title
Acute dengue virus 2 infection in Gabonese patients is associated with an early innate immune response, including strong interferon alpha production
Abstract
Background: Dengue is now a leading cause of morbidity and mortality throughout the tropics. We conducted the first ex vivo study of dengue fever (DF) in African patients infected during the first Gabonese dengue virus 2 (DENV-2) outbreak in 2007, in order to investigate cytokine production, including the antiviral cytokine IFN-α, reported to be a potent inhibitor of DENV replication in vitro.Methods: Levels of 50 cytokines, chemokines and growth factors were measured in plasma from 36 patients with DENV-2 infection, and in uninfected controls, using Luminex multiplex technology. The results were interpreted according to the day of sampling after symptom onset. PBMC from six patients were also studied for T lymphocyte cell surface marker expression by flow cytometry.Results: Acute DENV-2 infection elicited high levels of several pro-inflammatory cytokines (IL-6 and IL-17), chemokines (MIF, RANTES, IP-10 and MCP-1) and growth factors (G-CSF, GM-CSF and VEGF-A). We also observed high levels of IFN-α for the first time in adult DF patients, and CD4+ and CD8+ T cell activation at symptom onset.Conclusion: Acute DENV-2 infection in African patients elicits a strong innate response involving IFN-α production, as well as an adaptive immune response. © 2010 Becquart et al; licensee BioMed Central Ltd.
