Publication: Establishment and characterization of a cholangiocarcinoma cell line (RMCCA-1) from a Thai patient
Issued Date
2006-10-28
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ISSN
10079327
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2-s2.0-33751010766
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Mahidol University
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SCOPUS
Bibliographic Citation
World Journal of Gastroenterology. Vol.12, No.40 (2006), 6500-6506
Suggested Citation
Panthip Rattanasinganchan, Kawin Leelawat, Sa Ard Treepongkaruna, Chintana Tocharoentanaphol, Somboon Subwongcharoen, Tuangporn Suthiphongchai, Rutaiwan Tohtong Establishment and characterization of a cholangiocarcinoma cell line (RMCCA-1) from a Thai patient. World Journal of Gastroenterology. Vol.12, No.40 (2006), 6500-6506. doi:10.3748/wjg.v12.i40.6500 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/23531
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Title
Establishment and characterization of a cholangiocarcinoma cell line (RMCCA-1) from a Thai patient
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Abstract
Aim: To establish and characterize a new cell line derived from peripheral cholangiocarcinoma of a Thai patient. Methods: The peripheral cholangiocarcinoma specimen surgically obtained from the patient was aseptically processed by washing and mincing before culturing in Ham's F12 medium containing 10% fetal bovine serum. After 3 mo, when the cell line has become homogeneous and stabilized, several features were investigated, including growth characteristics, immunofluorescence staining for cytokeratins, expression of tumor markers, chromosomal analysis by G-banding and multicolour fluorescence in situ hybridization (mFISH), in vitro migration and invasion characteristics. Results: The RMCCA-1 cell line has been established. These cells proliferated as a monolayer with a population doubling time of 48 h. Immunofluorescence staining showed positive staining for human cytokeratin 7 and 19 verifying the biliary epithelial origin. RMCCA-1 secreted carbohydrate antigen 19-9 (CA19-9), but insignificant levels of carcinoembryonic antigen (CEA) and α-fetoprotein (AFP). Chromosome analysis identified aneuploidy karyotypes with a modal chromosome number of 59. RMCCA-1 exhibited a low level of in vitro invasiveness, but a high degree of motility. The cell line exhibited a significant number of chromosomal aberrations as shown by mFISH and G-banding methods. Conclusion: A new cell line derived from peripheral cholangiocarcinoma of a Thai patient has been established. This cell line shows a low level of in vitro invasiveness, but a high degree of motility. It will serve as a valuable tool for further studies on tumor biology, molecular pathogenesis, metastatic mechanism and response to therapeutic drugs of cholangiocarcinoma. © 2006 The WJG Press. All rights reserved.