Publication: The fibrinogen-like domain of FREP1 protein is a broad-spectrum malaria transmission-blocking vaccine antigen
Issued Date
2017-07-14
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ISSN
1083351X
00219258
00219258
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2-s2.0-85023605528
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Biological Chemistry. Vol.292, No.28 (2017), 11960-11969
Suggested Citation
Guodong Niu, Caio França, Genwei Zhang, Wanlapa Roobsoong, Wang Nguitragool, Xiaohong Wang, Jetsumon Prachumsri, Noah S. Butler, Jun Li The fibrinogen-like domain of FREP1 protein is a broad-spectrum malaria transmission-blocking vaccine antigen. Journal of Biological Chemistry. Vol.292, No.28 (2017), 11960-11969. doi:10.1074/jbc.M116.773564 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/41840
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Title
The fibrinogen-like domain of FREP1 protein is a broad-spectrum malaria transmission-blocking vaccine antigen
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Abstract
© 2017 by The American Society for Biochemistry and Molecular Biology, Inc. FREP1 in mosquito midguts facilitates Plasmodium falciparum parasite transmission. The fibrinogen-like (FBG) domain of FREP1 is highly conserved (>90% identical) among Anopheles species from different continents, suggesting that anti-FBG antibodies may block malaria transmission to all anopheline mosquitoes. Using standard membrane-feeding assays, anti-FREP1 polyclonal antibodies significantly blocked transmission of Plasmodium berghei and Plasmodium vivax to Anopheles gambiae and Anopheles dirus, respectively. Furthermore, in vivo studies of mice immunized with FBG achieved >75% blocking efficacy of P. berghei to A. gambiae without triggering immunopathology. Anti-FBG serum also reduced >81% of P. falciparum infection to A. gambiae. Finally, we showed that FBG interacts with Plasmodium gametocytes and ookinetes, revealing the molecular mechanism of its antibody transmission-blocking activity. Collectively, our data support that FREP1-mediated Plasmodium transmission to mosquitoes is a conserved pathway and that targeting the FBG domain of FREP1 will limit the transmission of multiple Plasmodium species to multiple Anopheles species.