Publication: Melatonin ameliorates Aβ<inf>42</inf>-induced alteration of βAPP-processing secretases via the melatonin receptor through the Pin1/GSK3β/NF-κB pathway in SH-SY5Y cells
Issued Date
2018-05-01
Resource Type
ISSN
1600079X
07423098
07423098
Other identifier(s)
2-s2.0-85041714033
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of Pineal Research. Vol.64, No.4 (2018)
Suggested Citation
Vorapin Chinchalongporn, Mayuri Shukla, Piyarat Govitrapong Melatonin ameliorates Aβ<inf>42</inf>-induced alteration of βAPP-processing secretases via the melatonin receptor through the Pin1/GSK3β/NF-κB pathway in SH-SY5Y cells. Journal of Pineal Research. Vol.64, No.4 (2018). doi:10.1111/jpi.12470 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/45175
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Melatonin ameliorates Aβ<inf>42</inf>-induced alteration of βAPP-processing secretases via the melatonin receptor through the Pin1/GSK3β/NF-κB pathway in SH-SY5Y cells
Other Contributor(s)
Abstract
© 2018 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd Melatonin is involved in the physiological regulation of the β-amyloid precursor protein (βAPP)-cleaving secretases which are responsible for generation of the neurotoxic amyloid beta (Aβ) peptide, one of the hallmarks of Alzheimer's disease (AD) pathology. In this study, we aimed to determine the underlying mechanisms of this regulation under pathological conditions. We establish that melatonin prevents Aβ42-induced downregulation of a disintegrin and metalloproteinase domain-containing protein 10 (ADAM10) as well as upregulation of β-site APP-cleaving enzyme 1 (BACE1) and presenilin 1 (PS1) in SH-SY5Y cell cultures. We also demonstrate that the intrinsic mechanisms of the observed effects occurred via regulation of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) and glycogen synthase kinase (GSK)-3β as melatonin reversed Aβ42-induced upregulation and nuclear translocation of NF-κBp65 as well as activation of GSK3β via its receptor activation. Furthermore, specific blocking of the NF-κB and GSK3β pathways partially abrogated the Aβ42-induced reduction in the BACE1 and PS1 levels. In addition, GSK3β blockage affected α-secretase cleavage and modulated nuclear translocation of NF-κB. Importantly, our study for the first time shows that peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 (Pin1) is a crucial target of melatonin. The compromised levels and/or genetic variation of Pin1 are associated with age-dependent tau and Aβ pathologies and neuronal degeneration. Interestingly, melatonin alleviated the Aβ42-induced reduction of nuclear Pin1 levels and preserved the functional integrity of this isomerase. Our findings illustrate that melatonin attenuates Aβ42-induced alterations of βAPP-cleaving secretases possibly via the Pin1/GSK3β/NF-κB pathway.