Publication:
Expression and purification of 30 kilodalton protein antigen of ARA <sup>-</sup>Burkholderia pseudomallei

Simple item page
dc.contributor.authorUtane Rungpanichen_US
dc.contributor.authorSupinya Pongsunken_US
dc.contributor.authorTakayuki Ezakien_US
dc.contributor.authorPattama Ekpoen_US
dc.contributor.otherMahidol Universityen_US
dc.contributor.otherSrinakharinwirot Universityen_US
dc.contributor.otherGifu Universityen_US
dc.date.accessioned2018-09-13T07:04:45Z
dc.date.available2018-09-13T07:04:45Z
dc.date.issued2009-03-01en_US
dc.description.abstractThe 30 kDa protein of B. pseudomallei is found in virulent Ara- but not avirulent Ara+ strain. The gene was cloned in Escherichia coli JM105 employing pInIII-C2 vector. The open reading frame was 870 nucleotides with a guanine plus cytosine content of 69.9%. Arginine was the most abundant amino acid in the protein, having a PI of 12.65. Nucleotide sequence of the gene was 96% identical to B. pseudomallei 1710b chromosome II sequence CP000125.1, encoding an oxidoreductase of the short chain dehydrogenase/ reductase family. The 30 kDa antigen was expressed as a maltose-fusion protein with a yield of 5.25 mg/l of bacterial culture.en_US
dc.identifier.citationSoutheast Asian Journal of Tropical Medicine and Public Health. Vol.40, No.2 (2009), 295-301en_US
dc.identifier.issn01251562en_US
dc.identifier.other2-s2.0-63149121299en_US
dc.identifier.urihttps://repository.li.mahidol.ac.th/handle/20.500.14594/28178
dc.rightsMahidol Universityen_US
dc.rights.holderSCOPUSen_US
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=63149121299&origin=inwarden_US
dc.subjectMedicineen_US
dc.titleExpression and purification of 30 kilodalton protein antigen of ARA <sup>-</sup>Burkholderia pseudomalleien_US
dc.typeArticleen_US
dspace.entity.typePublication
mu.datasource.scopushttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=63149121299&origin=inwarden_US

Files

Collections

Scopus 2006-2010