Publication: Virulence assay of rhizoid and non-rhizoid morphotypes of Flavobacterium columnare in red tilapia, Oreochromis sp., fry
Issued Date
2016-06-01
Resource Type
ISSN
13652761
01407775
01407775
Other identifier(s)
2-s2.0-84929094618
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Fish Diseases. Vol.39, No.6 (2016), 649-655
Suggested Citation
H. T. Dong, S. Senapin, B. Lafrentz, C. Rodkhum Virulence assay of rhizoid and non-rhizoid morphotypes of Flavobacterium columnare in red tilapia, Oreochromis sp., fry. Journal of Fish Diseases. Vol.39, No.6 (2016), 649-655. doi:10.1111/jfd.12385 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/40892
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Title
Virulence assay of rhizoid and non-rhizoid morphotypes of Flavobacterium columnare in red tilapia, Oreochromis sp., fry
Author(s)
Abstract
© 2016 John Wiley & Sons Ltd. Numerous isolates of Flavobacterium columnare were previously recovered from red tilapia, Oreochromis sp., exhibiting columnaris-like disease in Thai farms, and the phenotypic and genetic characteristics were described. The objective of this study was to determine the virulence of two morphotypes (rhizoid and non-rhizoid colonies) of F. columnare and to determine their ability to adhere to and persist in red tilapia fry. The results showed that the typical rhizoid isolate (CUVET1214) was a highly virulent isolate and caused 100% mortality within 24 h following bath challenge of red tilapia with three different doses. The non-rhizoid isolate (CUVET1201) was avirulent to red tilapia fry. Both morphotypes adhered to and persisted in tilapia similarly at 0.5 and 6 h post-challenge as determined by whole fish bacterial loads. At 24 and 48 h post-challenge, fry challenged with the rhizoid morphotype exhibited significantly higher bacterial loads than the non-rhizoid morphotype. The results suggested that an inability of the non-rhizoid morphotype to persist in tilapia fry may explain lack of virulence.