Publication: Exploring the limits of ultrafast polymerase chain reaction using liquid for thermal heat exchange: A proof of principle
Issued Date
2010-12-27
Resource Type
ISSN
00036951
Other identifier(s)
2-s2.0-78650900333
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Applied Physics Letters. Vol.97, No.26 (2010)
Suggested Citation
George Maltezos, Matthew Johnston, Konstantin Taganov, Chutatip Srichantaratsamee, John Gorman, David Baltimore, Wasun Chantratita, Axel Scherer Exploring the limits of ultrafast polymerase chain reaction using liquid for thermal heat exchange: A proof of principle. Applied Physics Letters. Vol.97, No.26 (2010). doi:10.1063/1.3530452 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/29936
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Exploring the limits of ultrafast polymerase chain reaction using liquid for thermal heat exchange: A proof of principle
Abstract
Thermal ramp rate is a major limiting factor in using real-time polymerase chain reaction (PCR) for routine diagnostics. We explored the limits of speed by using liquid for thermal exchange rather than metal as in traditional devices, and by testing different polymerases. In a clinical setting, our system equaled or surpassed state-of-the-art devices for accuracy in amplifying DNA/RNA of avian influenza, cytomegalovirus, and human immunodeficiency virus. Using Thermococcus kodakaraensis polymerase and optimizing both electrical and chemical systems, we obtained an accurate, 35 cycle amplification of an 85-base pair fragment of E. coli O157:H7 Shiga toxin gene in as little as 94.1 s, a significant improvement over a typical 1 h PCR amplification. © 2010 American Institute of Physics.