Publication: Cytotoxicity, apoptosis and DNA damage induced by Alpinia galanga rhizome extract
Issued Date
2007-08-01
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ISSN
00320943
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2-s2.0-34547767098
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Mahidol University
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SCOPUS
Bibliographic Citation
Planta Medica. Vol.73, No.8 (2007), 748-754
Suggested Citation
P. Muangnoi, M. Lu, J. Lee, A. Thepouyporn, R. Mirzayans, X. C. Le, Michael Weinfeld, S. Changbumrung Cytotoxicity, apoptosis and DNA damage induced by Alpinia galanga rhizome extract. Planta Medica. Vol.73, No.8 (2007), 748-754. doi:10.1055/s-2007-981542 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/24156
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Title
Cytotoxicity, apoptosis and DNA damage induced by Alpinia galanga rhizome extract
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Abstract
Alpinia galanga, or galangal, has been a popular condiment used in Thai and Asian cuisine for many years. However, relatively little is known of the potential beneficial or adverse health effects of this spice. This study was conducted to analyze the capacity of galangal extract to induce cytotoxicity and DNA damage in six different human cell lines including normal and p53-inactive fibroblasts, normal epithelial and tumour mammary cells and a lung adenocarcinoma cell line. We deliberately focused on treatment with the crude aqueous extract of galangal rhizomes, rather than compounds extracted into an organic solvent, to more closely reflect the mode of dietary consumption of galangal. The cell lines displayed a broad range of cytotoxicity. There was no evidence for preferential cytotoxicity of tumour cells, but there was an indication that p53-active cell lines may be more sensitive than their p53-inactive counterparts. The contribution of apoptosis to total cell killing was only appreciable after exposure to 300 μg/mL of extract. Apoptosis appeared to be independent of p53 expression. Exposure to as little as 100 μg/mL galangal extract generated a significant level of DNA single-strand breaks as judged by the single-cell gel electrophoresis technique (comet assay). The three major UV-absorbing compounds in the aqueous extract were identified by mass spectrometry as 1′-acetoxychavicol acetate and its deacetylated derivatives. However, when tested in A549 human lung adenocarcinoma cells, these compounds were not responsible for the cytotoxicity induced by the complete aqueous extract. © Georg Thieme Verlag KG Stuttgart.
