Publication: The Listeria monocytogenes σ<sup>B</sup>regulon and its virulence-associated functions are inhibited by a small molecule
Issued Date
2011-11-01
Resource Type
ISSN
21507511
Other identifier(s)
2-s2.0-84855212172
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
mBio. Vol.2, No.6 (2011)
Suggested Citation
M. Elizabeth Palmer, Soraya Chaturongakul, Martin Wiedmann, Kathryn J. Boor The Listeria monocytogenes σ<sup>B</sup>regulon and its virulence-associated functions are inhibited by a small molecule. mBio. Vol.2, No.6 (2011). doi:10.1128/mBio.00241-11 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/11967
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
The Listeria monocytogenes σ<sup>B</sup>regulon and its virulence-associated functions are inhibited by a small molecule
Other Contributor(s)
Abstract
The stress-responsive alternative sigma factor σ B is conserved across diverse Gram-positive bacterial genera. In Listeria monocytogenes, σ B regulates transcription of > 150 genes, including genes contributing to virulence and to bacterial survival under host-associated stress conditions, such as those encountered in the human gastrointestinal lumen. An inhibitor of L. monocytogenes σ B activity was identified by screening ~57,000 natural and synthesized small molecules using a high-throughput cell-based assay. The compound fluoro-phenyl-styrene-sulfonamide (FPSS) (IC 50 = 3.5 μM) downregulated the majority of genes previously identified as members of the σ B regulon in L. monocytogenes 10403S, thus generating a transcriptional profile comparable to that of a 10403S ΔsigB strain. Specifically, of the 208 genes downregulated by FPSS, 75% had been identified previously as positively regulated by σ B . Downregulated genes included key virulence and stress response genes, such as inlA, inlB, bsh, hfq, opuC, and bilE. From a functional perspective, FPSS also inhibited L. monocytogenes invasion of human intestinal epithelial cells and bile salt hydrolase activity. The ability of FPSS to inhibit σ B activity in both L. monocytogenes and Bacillus subtilis indicates its utility as a specific inhibitor of σ B across multiple Gram-positive genera. © 2011 Palmer et al.