Publication: Immune response in rabbits to dengue viral proteins.
Issued Date
1990-12-01
Resource Type
ISSN
01251562
Other identifier(s)
2-s2.0-0025655898
Rights
Mahidol University
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SCOPUS
Bibliographic Citation
The Southeast Asian journal of tropical medicine and public health. Vol.21, No.4 (1990), 621-629
Suggested Citation
V. Churdboonchart, N. Bhamarapravati, K. Sirisidhi Immune response in rabbits to dengue viral proteins.. The Southeast Asian journal of tropical medicine and public health. Vol.21, No.4 (1990), 621-629. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/15984
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Title
Immune response in rabbits to dengue viral proteins.
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Abstract
Antisera to all types of dengue and Japanese encephalitis (JE) viruses were raised in rabbits. The first set of rabbits was immunized with crude antigens prepared by a sucrose acetone extraction. The first generation of antisera demonstrated antibody activity towards the group specific flaviviruses, without unwanted antibody activity towards the mouse brain material. Antibody activity towards E, NS3, NS5 and NS1 could be observed by western blot/immunoenzymatic assay. Another set of rabbits was immunized with the precipitin complexes formed between antisera raised against each type of dengue, or JE viruses, and their homologous antigens. Each rabbit serum was screened again by the western blot/immunoenzymatic method, and was absorbed with other types of dengue viruses until the specific activity towards the immunized viral antigen was obtained. The last 2 bands detected on the viral antigen strip were the doublet of protein bands at Mr 67 and 71 kDa, which are the NS3 protein. The specific polyclonal antisera obtained can be used with other tests as well as the monoclonal antibody. Since absorption can lead to type specific antisera, this NS3 protein must be quite unique, it processes a type specific determinant (s) and deserves further study as a target molecule at the polypeptide level as well as at the RNA level.