Publication: Effects of DHA-enriched hen egg yolk and L-cysteine supplementation on quality of cryopreserved boar semen
Issued Date
2009-09-21
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ISSN
17457262
1008682X
1008682X
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2-s2.0-70349162026
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Mahidol University
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SCOPUS
Bibliographic Citation
Asian Journal of Andrology. Vol.11, No.5 (2009), 600-608
Suggested Citation
Panida Chanapiwat, Kampon Kaeoket, Padet Tummaruk Effects of DHA-enriched hen egg yolk and L-cysteine supplementation on quality of cryopreserved boar semen. Asian Journal of Andrology. Vol.11, No.5 (2009), 600-608. doi:10.1038/aja.2009.40 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/27927
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Title
Effects of DHA-enriched hen egg yolk and L-cysteine supplementation on quality of cryopreserved boar semen
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Abstract
The objective of the present study was to determine the effects of docosahexaenoic acid (DHA)-enriched hen egg yolks and L-cysteine supplementation on the qualities of the cryopreserved boar semen. A total of 15 ejaculates from 5 Pietrain boars were divided into 4 groups according to the compositions of the freezing extenders used, that is, normal hen egg yolk (group I), DHA-enriched hen egg yolk (group II), normal hen egg yolk with 5 mmol L-1 of cysteine supplementation (group III) and DHA-enriched hen egg yolk with 5 mmol L-1 of cysteine supplementation (group IV). The semen was cryopreserved using controlled rate freezer and was thawed at 50°C for 12 s. Progressive motility, sperm viability, acrosome integrity and functional integrity of sperm plasma membrane of the post-thawed semen were evaluated. The supplementation of L-cysteine in the freezing extender alone (group III) improved progressive motility (P < 0.05), and the supplementation of L-cysteine in combination with DHA-enriched hen egg yolk (group IV) improved both progressive motility (P < 0.05) and acrosome integrity (P < 0.01). The use of DHA-enriched hen egg yolk alone (group II) did not enhance any of the post-thawed semen qualities (P > 0.05). In conclusion, the supplementation of antioxidant L-cysteine alone or in combination with DHA-enriched hen egg yolk significantly improved the post-thawed semen qualities, especially progressive motility and acrosome integrity.