Publication: Proteomic analyses in fast and slow-cleaving bovine embryos – a search for potential markers of healthy pre-implantation embryos
7
Issued Date
2021-01-01
Resource Type
ISSN
01253395
Other identifier(s)
2-s2.0-85119010833
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Songklanakarin Journal of Science and Technology. Vol.43, No.5 (2021), 1292-1298
Suggested Citation
Preeyalak Konongbua, Atthaboon Watthammawut, Sittiruk Roytrakul, Monsicha Somrit, Somluk Asuvapongpatana, Wattana Weerachatyanukul Proteomic analyses in fast and slow-cleaving bovine embryos – a search for potential markers of healthy pre-implantation embryos. Songklanakarin Journal of Science and Technology. Vol.43, No.5 (2021), 1292-1298. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/79395
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Proteomic analyses in fast and slow-cleaving bovine embryos – a search for potential markers of healthy pre-implantation embryos
Abstract
Selection of embryos with potentially successful development is paramount to ensuring their viability and growth. While morphological assessment has been the mainstay of embryo selection, the identification of proteins that are expressed in the more reproductively successful fast-cleaving embryos as opposed to slow-cleaving ones may serve to reduce visual assessment of subjectivity in bovine. Through the use of proteomic mass spectrometric techniques, we found that 2387 proteins were commonly identified in both fast and slow-cleaving embryos, while 464 proteins were differentially identified. Slow-cleaving embryos showed a higher percentage of identified proteins involved in apoptotic processes, DNA repair and stress responses, while fast-cleaving bovine embryos showed a higher percentage of proteins identified that are involved in cell metabolism, gene transcription, and signal transduction. These differential protein expressions identified in the fast-and slow-cleaving embryos may serve as alternative potential markers of healthy pre-implantation embryos and improve overall bovine reproductive rate.