Publication: Amperometric acetylcholine biosensor based on graphene-PEDOT:PSS modified electrode
Issued Date
2015-01-01
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2-s2.0-84922978468
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Mahidol University
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SCOPUS
Bibliographic Citation
BMEiCON 2014 - 7th Biomedical Engineering International Conference. (2015)
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Piyawat Pitikultham, Chakrit Sriprachuabwong, Pornpimol Sritongkham Amperometric acetylcholine biosensor based on graphene-PEDOT:PSS modified electrode. BMEiCON 2014 - 7th Biomedical Engineering International Conference. (2015). doi:10.1109/BMEiCON.2014.7017408 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/35946
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Title
Amperometric acetylcholine biosensor based on graphene-PEDOT:PSS modified electrode
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Abstract
© 2014 IEEE. A promising acetylcholine biosensor based on graphene-PEDOT:PSS (GR-PEDOT:PSS) and bienzyme, choline oxidase (ChOx) and acetylcholine esterase (AChE), modified electrode was successfully fabricated. GR-PEDOT:PSS ink was directly dropped on the screen printed carbon electrode (SPE), and then ChOx and AChE in the optimized loading ratio were immobilized on GR-PEDOT:PSS/SPE by the 10% glutar-aldehyde (GA) vapor cross-linked for 8 minutes. 1% Nafion was coated on the outermost layer of AChE-ChOx/GR-PEDOT:PSS/SPE for performing as a protective film. For amperometric responding, our acetylcholine biosensor showed a low limit detection, high linearity and sensitivity, which were 50 μM, 50 to 250 μM and 486.1 nA/mM, respectively, at -200 mV versus Ag/AgCl reference electrode. Moreover, this biosensor has a simple preparation, low-cost fabrication, and stable detection, thus it is a novel tool for acetylcholine determination.