Publication: LINE-1 and Alu hypomethylation in mucoepidermoid carcinoma
2
Issued Date
2013
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eng
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Mahidol University
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BioMed Central
Bibliographic Citation
BMC Clinical Pathology. Vol.13, (2013), 10
Suggested Citation
Porntipa Sirivanichsuntorn, Somboon Keelawat, Kittipong Danuthai, Apiwat Mutirangura, Keskanya Subbalekha, Nakarin Kitkumthorn LINE-1 and Alu hypomethylation in mucoepidermoid carcinoma. BMC Clinical Pathology. Vol.13, (2013), 10. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/2767
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Title
LINE-1 and Alu hypomethylation in mucoepidermoid carcinoma
Abstract
Background: Mucoepidermoid carcinoma (MEC) can be classified into low-, intermediate-, and high-grade tumors
based on its histological features. MEC is mainly composed of three cell types (squamous or epidermoid, mucous
and intermediate cells), which correlates with the histological grade and reflects its clinical behavior. Most cancers
exhibit reduced methylation of repetitive sequences such as Long INterspersed Element-1 (LINE-1) and Alu
elements. However, to date very little information is available on the LINE-1 and Alu methylation status in MEC. The
aim of this study was to investigate LINE-1 and Alu element methylation in MEC and compare if key differences in
the methylation status exist between the three different cell types, and adjacent normal salivary gland cells, to see if
this may reflect the histological grade.
Methods: LINE-1 and Alu element methylation of 24 MEC, and 14 normal salivary gland tissues were compared
using Combine Bisulfite Restriction Analysis (COBRA). Furthermore, the three different cell types from MEC samples
were isolated for enrichment by laser capture microdissection (LCM), essentially to see if COBRA was likely to
increase the predictive value of LINE-1 and Alu element methylation.
Results: LINE-1 and Alu element methylation levels were significantly different (p<0.001) between the cell types,
and showed a stepwise decrease from the adjacent normal salivary gland to the intermediate, mucous and
squamous cells. The reduced methylation levels of LINE-1 were correlated with a poorer histological grade. In
addition, MEC tissue showed a significantly lower level of LINE-1 and Alu element methylation overall compared to
normal salivary gland tissue (p<0.001).
Conclusions: Our findings suggest that LINE-1 methylation differed among histological grade mucoepidermoid
carcinoma. Hence, this epigenetic event may hold value for MEC diagnosis and prognostic prediction.